尿中乙型肝炎病毒DNA检测对小儿乙肝相关性肾炎诊断价值研究  

Study on correlation of detection of HBV-DNA in urine to diagnosis of children with hepatitis B virus associated glomerulonephritis

作　　者：何小解[1] 易著文[1] 何庆南[1] 党西强[1] 吴小川[1] 赵维玲[1] 

机构地区：[1]中南大学湘雅医学院第二附属医院小儿肾脏病研究室,长沙410011

出　　处：《中国实用儿科杂志》2002年第2期89-91,共3页Chinese Journal of Practical Pediatrics

基　　金：湖南省科委资助项目 (NO :98-SSY -2 0 17)

摘　　要：目的　探讨尿中乙肝病毒DNA(HBV DNA)的检测对诊断小儿乙肝病毒相关性肾炎 (HBV GN)的意义。方法　应用聚合酶链式反应 (PCR)、免疫荧光 (IF)及原位杂交法 (ISH)检测患儿血清和尿中HBV DNA及肾活检石蜡包埋组织切片中HBV抗原与HBV DNA的存在状况。结果　 32例患儿经血清HBV DNA与肾组织中HBV抗原检测 ,符合HBV GN诊断的 19例 ,其中 11例经ISH检测发现肾活检石蜡包埋组织切片中HBV DNA阳性 ,13例尿HBV DNA阳性 ,阳性率分别为 5 7 9%、6 8 5 %。结论　检测肾小球疾病患儿尿中HBV DNA可协助HBVObjective To study the significance of detection of HBV-DNA in urine in diagnosis of hepatitis B virus associated glomerulonephritis(HBV-GN).Methods To detect respectively HBV-DNA in serum and in urine,and HBV-Ag and HBV-DNA in paraffin-embedded renal biopsy tissue by polymerase chain reaction(PCR),immuno fluorescence(IF),and in situ hybridization(ISH).Results 19 from 32 objects with HBV-GN were diagnosed by detecting HBV-DNA in serum and HBV-Ag in paraffin-embedded renal biopsy tissue . HBV-DNA were positive in urine in 13 out of 19 objects and in 11 out of 19 objects in paraffin-embedded renal biopsy tissue, the positive rate was 68.5% and 57.9% respectively.Conclusion Detecting HBV-DNA in urine for patients with nephrosis may be helpful in diagnosis of HBV-GN.

关 键 词：乙型肝炎病毒 肾炎 原位杂交 聚合酶链反应 脱氧核糖核酸 DNA 儿童 尿 

分 类 号：R725.126.2[医药卫生—儿科] R726.923[医药卫生—临床医学]