基质金属蛋白酶-1及白细胞介素-1αmRNA在中耳胆脂瘤上皮中的表达  

The expression of matrix metalloproteinase-1 and interleukin-1-alpha messenger ribonucleic acid in human middle ear cholesteatoma

作　　者：刘建治[1] 林国经[1] 黄建民[1] 江张舟[1] 

机构地区：[1]福建医科大学附属协和医院耳鼻咽喉科,福州350001

出　　处：《中华耳鼻咽喉科杂志》2002年第1期30-33,T003,共5页Chinese Journal of Otorhinolaryngology

摘　　要：目的　探讨基质金属蛋白酶 (matrixmetalloproteinases ,MMP1)及其组织抑制剂 (tissueinhibitorofmetalloproteinases,TIMP1)和白细胞介素 1αmRNA(interleukin 1 alphamessengerribonucleicacid ,IL 1αmRNA)在中耳继发性胆脂瘤上皮的表达 ,分析其表达在胆脂瘤上皮溶骨性破坏中的作用。方法　分别应用免疫组化SP染色法和原位杂交技术检测MMP1、TIMP1和IL 1α在 32例胆脂瘤和 14例胆脂瘤患者外耳道皮肤中的表达情况。结果　MMP1在 32例胆脂瘤上皮各层细胞均存在较强表达 ,定位在胞膜和胞浆 ,外耳道皮肤表达较弱 ;两种组织MMP1阳性表达的平均 ( x±s,下同 )吸光度 (A值 )分别为 (2 0 18 2 6± 174 89)和 (142 8 35± 12 3 39) ,差异有极显著性 (t=10 6 9,P <0 0 1) ;TIMP1在两者中未见明显表达 ;外耳道皮肤仅基底层细胞可见散在IL 1αmRNA阳性表达细胞 ,平均吸光度 (A值 )为 (5 6 6 2 7± 148 94) ,胆脂瘤上皮除基底层细胞外 ,部分区域基底上层细胞也见阳性表达 ,平均吸光度(A值 )为 938 91± 2 2 3 46 ,差异有极显著性 (t=5 0 3,P <0 0 1)。结论　①MMP1在胆脂瘤上皮中高表达 ,并提示有MMP1和TIMP1表达失衡 ,与胆脂瘤骨吸收特性有关。②胆脂瘤上皮高表达的ILObjective To explore the expression of matrix metalloproteinase-1(MMP1), tissue inhibitor of metalloproteinase-1(TIMP1) and interleukin-1-alpha messenger ribonucleic acid(IL-1α mRNA) in acquired middle ear cholesteatoma, and to evaluate their roles in the molecular mechanisms of bone resorption. Methods Tissue specimens from 32 cases of acquired middle ear cholesteatoma and 14 cases of external ear skin were examined by immunohistochemical S-P method for MMP1 and TIMP1, and by in situ hybridization for IL-1α mRNA. Results All 32 samples of cholesteatoma showed a stronger expression of MMP1 than the external ear skin. The integral absorbency of MMP1 in the two types of tissues were 2 018.26±174.89 and 1 428.35±123.39, respectively, with statistically significant difference between them. Neither of the two types of epithelial cells showed a remarkable expression of TIMP1. The cells hybridized for the antisence probes IL-1α mRNA were only confined to the basal layer; in cholesteatoma, besides the basal cell layers ,keratinocytes of the suprabasal cell layers were also found to contain specific hybridizations. The level of IL-1α mRNA measured in cholesteatoma was significantly higher than that in the external ear skin. Conclusion The overexpression of MMP1 and a derailment of the normally controlled MMPs -TIMPs system could play an active role in the molecular mechanisms of cholesteatoma invasion into the bone. The upregulation of IL-1α might contribute to the increasing secreations of MMP1 in cholesteatoma.

关 键 词：中耳胆脂瘤 金属蛋白酶类 白细胞介素类 金属蛋白酶类组织抑制剂 MMP1 TIMP1 IL-1α mRNA 

分 类 号：R764.22[医药卫生—耳鼻咽喉科]