肝素结合的成纤维细胞生长因子促进鼠皮肤烧伤愈合的研究  

A Study of Heparin Binding Fibroblast Growth Factor Improving Burned Wound Healing in Mouse Skin

作　　者：董玉兰[1] 李永军[1] 王跃中[1] 张亚佳[1] 

机构地区：[1]中国医科大学基础医学院实验病理学研究室,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第1期3-4,8,共3页Journal of China Medical University

基　　金：沈阳市科委社发基金资助项目 (98493 40 5 14 )

摘　　要：目的 :探讨肝素结合的成纤维细胞生长因子 (FGF)对烧伤愈合的促进作用。方法 :于小鼠背部皮肤用2 0W电烙 ,电灼 2 0s,烙成直径 0 .5cm圆形II度烧伤伤口 ,实验组用FGF(含FGF 30 0 μg/ml,肝素 10 0 μg/ml) ,对照组用生理盐水 ,每天换药 1次。术后 1,3,5 ,7,10d处死动物 ,观察伤口愈合情况。结果 :FGF明显促进烧伤伤口处毛细血管、成纤维细胞增生 (P <0 .0 1) ,胶原蛋白的生成 ,肉芽组织的增生及皮肤的修复。实验组较对照组愈合快 4～ 5d。结论 :肝素结合的FGF促进烧伤伤口的肉芽组织增生 ,胶原蛋白及基质的生成 。Objective: To observe the improving effect of fibroblast growth factor (FGF) on burned wound healing. Methods: Two wounds were made by anelectric cautery at 20 W, lasting 20 s on the back of every mouse. One for experiment and the other for control.The circular wounds were II degree burn,and diameter was 0.5 cm.The wounds of the experimental group were covered with 0.5 ml FGF solution (contented FGF 300 μg/ml,heparin 100 μg/ml),whereas the wounds of the control group were covered with 0.5 ml 0.9% NaCl solution.All of the wounds were dressed by sterilized gauze, and received the same treatment once a day. After 1,3,5,7,10 days, the mice in every group were killed and the tissues of the wounds were collected and prepared for microscopic examination. Results:The results showed that FGF promoted the growth of capillaries and fibroblasts in the experiment group significantly. The collagen and granulation tissue were markedly increased, and the wound healing of experiment group was faster than that of the control group by 4-5 days. Conclusion:FGF purified from natural substance may play an important role in improving wound healing.

关 键 词：成纤维细胞生长因子 肉芽组织 烧伤愈合 

分 类 号：R644[医药卫生—外科学]