PGF_2α类抗青光眼药Unoprostone对培养猴睫状肌细胞中胶原Ⅲ、Ⅳ及MMP-1表达的影响  

Effect of Prostaglandin F_2α Analogue-Unoprostone on the Expression of CollagenⅢ,Ⅳ and Matrix Metalloproteinase-1 in Cultured Monkey Ciliary Muscle Cell

作　　者：金明[1] 洪晶[1] 吴景天[1] 

机构地区：[1]中国医科大学第一临床学院眼科,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第1期40-42,共3页Journal of China Medical University

摘　　要：目的 :探讨前列腺素F2 α(prostaglandinF2 α ,PGF2 α)类抗青光眼药Unoprostone对猴的降眼压作用机制是否与睫状肌中基质金属蛋白酶 (matrixmetalloproteinase ,MMP)及细胞外基质 (extracellularmatrix ,ECM)的表达有关。方法 :采用免疫细胞化学方法对培养猴睫状肌细胞中胶原Ⅰ ,Ⅲ ,Ⅳ及MMP 1的分布进行观察。比较 10 -6mol/LPGF2 α、Unoprostone代谢物M1及M2 对睫状肌细胞作用前后胶原Ⅲ ,Ⅳ及MMP 1的染色变化。结果 :胶原Ⅲ ,Ⅳ及MMP 1在培养猴睫状肌细胞内外分布各异 ,胶原Ⅰ未见明显染色 ;10 -6mol/LPGF2 α、Unoprostone代谢物M1及M2 分别使猴睫状肌细胞中胶原Ⅲ ,Ⅳ减少 ,MMP 1表达增强。结论 :Unoprostone增强猴睫状肌MMP表达 ,引起ECM降解加强 ,表明其降眼压机制为使睫状肌束间隙增大 。Objective: To evaluate whether the ocular hypotensive mechanism of prostaglandin F 2α(PGF 2α) analogue Unoprostone is related to the expression of matrix metalloproteinase (MMP) and extracellular matrix (ECM) in ciliary muscle. Methods: Monkey ciliary muscle cells were cultured. The distribution of collagen I, III, IV and MMP 1 in the cultured cells was observed using immunocytochemistic ethod. The staining change of collagen III, IV and MMP 1 in cultured ciliary muscle cells before and after exposing to 10 -6 mol/L PGF 2α, Unoprostone metabolites M 1 and M 2 was compared. Results: The distribution of collagen I, III, IV and MMP 1 in ciliary muscle cells was different. The staining of collagen Ⅲ, Ⅳ was reduced when exposed to 10 -6 mol/L PGF 2α, Unoprostone metabolites M 1 and M 2, while the staining after exposing to MMP 1 was increased. Conclusion: These results suggest a role for Unoprostone in the expression of MMP and remodeling of ECM in ciliary muscle. A Unoprostone induced change in ECM might augment the flow of aqueous humour through the ciliary muscle bundles of the uveoscleral pathway.

关 键 词：猴 睫状肌细胞 胶原Ⅲ 胶原Ⅳ 基质金属蛋白酶-1 Unoprostone 葡萄膜巩膜房水流出 

分 类 号：R988.1[医药卫生—药品]