高效液相色谱法测定盐酸安非拉酮在中毒家兔体内的分布研究  

Study on distribution of amfepramone hydrochloride in acute poisoning rabbits organs

作　　者：井连平[1] 董伟林[2] 洪燕敏[1] 王增亮[3] 任新生[4] 

机构地区：[1]天津医科大学法医教研室,300070 [2]天津医科大学实验中心 [3]天津市刑事科学技术研究所 [4]天津市天和医院

出　　处：《天津医科大学学报》2001年第4期504-506,共3页Journal of Tianjin Medical University

摘　　要：目的 :采用HPLC法测定盐酸安非拉酮在急性中毒家兔体内的分布情况。方法 :家兔按150mg/kg剂量灌胃造成急性中毒4h ,用固相萃取及HPLC法测定其体内药物浓度。结果 :盐酸安非拉酮在家兔体内脑、心、肺、脾、肝、肾、胆汁、血及尿中的平均浓度分别为6.55±3.05、31.36±5.53、12.14±2.04、14.57±0.78、63.17±4.53、25.11±3.05、45.56±2.43、71.67±4.82、5.12±2.09( g/ml或 g/g)。结论 :盐酸安非拉酮在急性中毒家兔体内的分布不均匀 ,血、肝、胆汁是临床急性中毒病人毒物鉴定和法医学检案中中毒者毒物分析的较好检材。Objective: A HPLC method has been developed to determine the distribution of amfepramone hydrochloride in poisoned rabbit's organs.Methods:Four hours after P.O 150mg/kg,the concentrations of amfepramone hydrochloride in organs were determined by the solid-phase extraction with the high performance liquid chromatography(HPLC).Results:The concentrations of amfepramone hydrochloride in brain,heart,lung,spleen,liver,kidney,bile,blood,urine of poisoned rabbit were 6.55±3.05?31.36±5.53?12.14±2.04?14.57±0.78?63.17±4.53?25.11±3.05?45.56±2.43?71.67±4.82?5.12±2.09( g/ml or g/g)respectively. Conclusion:Distribution of amfepramone hydrochloride in poisoned rabbits is unsymmetrical.Blood,liver and bile are better samples for identification of points in emergency and analysis of amfepramone hydrochloride poisoning victim.

关 键 词：盐酸安非拉酮 高效液相色谱法 体内分布 急性中毒 

分 类 号：R991[医药卫生—毒理学]