参白口服液对病毒性心肌炎心肌细胞凋亡及其相关调控基因影响的研究  

Study on Effect of Myocardial Cell Apoptosis and Its Regulatory Gene in Viral Myocarditis by Shenbai Oral Liquor

作　　者：曹洪欣[1] 郭书文[2] 周令望[3] 朱海燕[1] 张华敏[1] 林晓峰[1] 栗仕铀[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]北京中医药大学东直门医院,北京100700 [3]哈尔滨医科大学,黑龙江哈尔滨150000

出　　处：《中国中医基础医学杂志》2001年第12期20-22,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助课题 (No.30 0 70 940 )

摘　　要：目的 :研究病毒性心肌炎 (VMC)与心肌细胞凋亡及其相关调控基因的关系及参白口服液的防治作用。方法 :给BALB C小鼠接种嗜鼠心肌柯萨奇B3 病毒 (CVB3m)诱发VMC ,在接种后 3、7、14、2 0、30d动态对照观察心肌细胞凋亡 (电镜、TUNEL)和相关调控基因C myc、Bcl 2蛋白的表达 (免疫组化技术 )情况。结果 :CVB3m 可引起VMC心肌细胞凋亡 ,C myc、Bcl 2蛋白的阳性表达支持心肌细胞凋亡情况。结论 :心肌细胞凋亡及其相关调控基因可能参与VMC的发生和发展 ,参白口服液有良好的防治作用。To suty the relationship of viral myocarditis (VMC) with myocardial cell apoptosis and its regulatory gene, and the effect of shenbai oral liquor on VMC. Method:The viral myocarditis in BALB/C mouse was obtained by coxsackie virus B 3m (CMB 3m ). The myocardial cell apoptosis and expressions of apoptosis regulatory gene c myc, Bcl 2 in various stages(the days of 3,7,14,20,30) of viral infection were detected by TDT/d UTP Nick End Labeling(TUNEL) and immunohistochemical method and electron microscope. Results:CVB 3m can Induse myocardial cell apoptosis of suckling Wistar rat. Increase in genetic expression of c myc and decrease in genetic expression of Bcl 2 in mouse myocardium support the finding of myocardial cell apoptosis. Conclusion:Myocardial cell apoptosis and its regulatory gene are partly related with development of VMC, and shenbai oral Liquor has significant functions of prevention and treatment.

关 键 词：病毒性心肌炎 参白口服液 细胞凋亡 基因表达 中医药疗法 

分 类 号：R259.422.1[医药卫生—中西医结合]