IL-4和IL-10调节嗜碱性粒细胞CXCR4表达及功能  

IL-4 and IL-10 regulate CXCR4 expression and functions in basophils

作　　者：黄保军[1] 谭锦泉[1] 胡春松[1] 王兴兵[1] 王明军[1] 李群[1] 罗畅民[1] 

机构地区：[1]安徽医科大学免疫学教研室,合肥230032

出　　处：《中华微生物学和免疫学杂志》2001年第6期585-589,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金 ( 39870 6 74);安徽省自然科学基金( 98436 6 30 );安徽省教育厅科学研究基金 ( 98JL0 6 3)资助项目

摘　　要：目的　研究IL 4和IL 10对人嗜碱性粒细胞上CXC趋化性细胞因子受体 4(CXCR4)表达和配体SDF 1α(Chemokinestromalcell derivedfactor 1alpha)功能的调节。方法　嗜碱性粒细胞的纯化技术 ,流式细胞术 ,实时定量逆转录PCR (RT PCR) ,胞内游离Ca2 +的变化 ,趋化性技术和组胺释放等方法进行测定与分析。结果　CXCR4大量表达在人外周血静息嗜碱性粒细胞上。IL 4可显著上调CXCR4蛋白和mRNA的表达 ,而IL 10则明显下调其表达。SDF 1α可通过CXCR4诱导嗜碱性粒细胞中游离Ca2 +增加 ,激活嗜碱性粒细胞使之产生趋化性游走并释放组胺。此活性可被抗CXCR4单抗所阻断。结论　IL 4和IL 10是CXCR4表达和功能重要的调节细胞因子 ,CXCR4 SDF 1α复合物相互作用对嗜碱性粒细胞的聚集和活化起着重要作用。Objective To investigate the regulation of CXCR4 and functions of its ligand SDF 1α in human basophils by IL 4 and IL 10. Methods Basophil purification, flow cytometry, RT PCR, intracellular free Ca 2+ detection, chemotaxis assay and histamine release assay. Results CXCR4, a receptor for SDF 1α, is abundantly expressed on human peripheral blood resting basophils. The CXCR4 expression was up or down regulated by T H2 associated cytokines IL 4 or IL 10. IL 4 or IL 10 could up or down regulate the CXCR4 mRNA expression in basophils. SDF 1α induced an increase of intercellular free Ca 2+ in basophils. SDF 1α activated basophils to display chemotaxis and histamine release by interaction with CXCR4 on the cells, which could be blocked by anti CXCR4 McAb. Conclusion IL 4 and IL 10 are both important regulators for CXCR4 expression which are important for the recruitment and activation of basophils. [

关 键 词：趋化因子 趋化因子受体 细胞活化 趋化作用 嗜碱性粒细胞 流式细胞术 

分 类 号：R392[医药卫生—免疫学]