苦参碱诱导白血病细胞株HL-60分化的实验研究  

Experimental Research of Sophocarpidine Inducing Leukemia Cell HL-60 into Differentiation

作　　者：罗文纪[1] 虞荣喜[1] 朱宁希[2] 

机构地区：[1]杭州市萧山区第一人民医院,浙江311200 [2]浙江省中医院,浙江310006

出　　处：《中国医药学报》2001年第5期21-24,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药管理局资助课题

摘　　要：目的 观察苦参碱对HL-60细胞的诱导分化作用。方法 采用体外细胞培养技术,从细胞增殖、形态学变化、NBT还原能力测定、非特异性酯酶染色及细胞表面分化抗原测定等方面观察。结果 苦参碱在12.5μg/ml—50μg/ml浓度范围内能明显抑制HL-60细胞的增殖,细胞向成熟粒系方向分化,NBT还原反应能力增强,α-NAE染色呈阴性,CD_(15)、CD_(11b)阳性率表达上升而CD_(33)阳性率表达降低,CD_(14)阳性率处理前后无变化。结论 苦参碱可能是一种良好的白血病细胞分化诱导剂,具有潜在的抗白血病作用。Aim was observed the inducing differentiation action of Sophocarpidine towards HL-60. Hie vitro cell cultivation technique was used to observe the cell proliferation, the morphological change, NBT reduction ability assay and nonspecific lipase staining. Results were showed that sophocarpidine can markedly inhibit cell HL-60 proliferation between the concentration of 12.5μg/ml and 50μg/ml, differentiation towards mature granulocyte series, that NBT reduction ability was improved,a-NAE staining was negative,CD15 CD11b positive rate expression increased and CD33 decreased, CD14 kept unchanged before and after the handling. Conclusion was that Sophocarpidine is possibly a fine leukemia cell inducing differentiation agent and has potential anti-leukemia action.

关 键 词：苦参碱 HL-60细胞 细胞分化 细胞分化诱导剂 白血病 中药药理 

分 类 号：R273[医药卫生—中西医结合]