脑尔康对老年痴呆小鼠学习记忆障碍及脑M受体的影响  

Effect of Nao Er Kang on Learning and Memory Disorders in Model Rats with Alzheimer's Diseases

作　　者：李玺[1] 张雪飞[2] 乔成林[1] 

机构地区：[1]西安交通大学第二医院,陕西710004 [2]青海医学院,青海810001

出　　处：《中国医药学报》2001年第5期24-27,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省"九五"科技攻关课题

摘　　要：目的:通过慢性铝中毒造成老年痴呆小鼠模型。观察复方中药脑尔康对小鼠学习记忆障碍的保护作用,探讨其对M受体含量的影响。方法:以跳台法进行学习训练,并记录潜伏期时间及错误次数。24小时后再测记忆成绩,行为测试结束后,动物迅速断头取脑,冰台分离海马及皮层,用ELISA法检测各自的M受体亚型含量。结果:模型组记忆潜伏期明显缩短(p<0.01),学习潜伏期明显延长(p<0.01),学习和记忆的错误次数明显增多(p<0.01),用药组记忆潜伏期均明显延长(p<0.01),学习潜伏期明显缩短(p<0.05或p<0.01),学习和记忆错误次数脑尔康三个剂量组亦明显减少(p<0.05),脑复康组记忆错误次数无明显差异(p>0.05);用药各组除小剂量组外均有提高模型小鼠皮层与海马M_1受体亚型含量的作用(p<0.05或p<0.01),但对M_2受体亚型含量皮层、海马均无显著性差异。结论:1.脑尔康对氯化铝所致小鼠学习记忆障碍具有保护作用。2.脑尔康能明显提高老年痴呆模型小鼠皮层及海马M_1受体含量。Objective: To observe the protective effect of Nao Er Kang on learning and memory disorders in model rats with Alzheimer's disease (AD) caused by aluminum chloride and to discuss Nao Er Kang effect on content of M-receptor. Methods: After administered of different drugs in different proups, rats were given training of step down test. Simutaneously,the latency period and error times were recorded,the learning results were observed 24 hours later. Others were rapidly decollated and brains were fetched, the contents of M-receptor subtypes were observed. Compared with control group, the memory's latency period in model group largely shortened(p< 0.01)and learning latency period prolonged obviously. In addition, the error times of learning and memory increased (p < 0.01) .In medicated group, the memory' s latency period obviously prolonged (p < 0.01) and learning latency period obviously shortened( p<0.05 orp<0.01). The error times of learning and memory in three medicated groups of Nao Er Kang shortened (p < 0.05).But there was no significant difference in three groups of Nao Er kang. The contents of M1 -receptor subtypes in the cortex and hippocampus of rats were largely improved except small dose group, but the contents of M2-receptor subtypes were no significant difference in these two areas. Conclusions: 1.Nao Er Kang can protect rats from learning and memory disorders. 2.Nao Er Kang can largely increase the content of M1-receptor in cortex and hippocampas in AD rats.

关 键 词：脑尔康 老年痴呆 小鼠 学习记忆障碍 M受体 中药学 

分 类 号：R285[医药卫生—中药学]