甲基一六O五降解菌J5的分离及其降解性状研究  

Study on the Isolation of Methyl Parathion Degrading Bacillus Strain J5 and the Characteristics of the Degradation.

作　　者：崔中利[1] 李顺鹏[1] 何健[1] 

机构地区：[1]南京农业大学微生物学系,江苏南京210095

出　　处：《农村生态环境》2001年第3期21-25,共5页Rural Eco-Environment

基　　金：科技部S863项目(SZ0 30 8)

摘　　要：从农药厂污水处理系统中分离到 1株降解甲基一六O五 (O ,O-二甲基 -O- 对硝基苯基 -硫代磷酸酯 ,简称MP)的芽胞杆菌 ,初步鉴定为蜡状芽胞杆菌 (Bacilluscereus)J5。J5能够高效降解MP ,但它不能利用MP作为唯一碳源生长 ,其代谢方式可能为共代谢。在有葡萄糖作为碳源的条件下 ,J5可以高效转化MP ,其转化效率可达 95 %以上。用薄层层析。From the wastewater treatment system of a pesticide plant, a Bacillus strain was isolated, capable of efficiently degrading methyl parathion (MP).It is preliminarily identified as Bacillus cereus J5.The bacteria,however,do not live on MP as the sole carbon and energy source.Experiment results suggest that J5 degrades MP by way of co metabolism.Growing in the medium with glucose as its carbon source, J5 can degrade MP by over 95%.Rate and metabolites of the degradation of MP by J5 were studied by means of TLC,ultra violate scanning and HPLC.

关 键 词：生物降解 甲基一六○五 共代谢 降解菌 分离 降解性状 农药残留 废水处理 生物修复 农药污染 

分 类 号：X832[环境科学与工程—环境工程] X172