人工组织神经移植物修复狗坐骨神经缺损后荧光素逆行追踪试验  

A STUDY OF FLUORESCENCE RETROGRADE TRACING ON REPAIR OF DOG SCIATIC NERVE GAP WITH ARTIFICIAL NERVE GRAFT

作　　者：王晓冬[1] 张沛云[1] 吕广明[1] 陈罡[1] 姚健[2] 

机构地区：[1]南通医学院分子神经生物学江苏省重点实验室,南通226001 [2]南通体臣卫校

出　　处：《南通医学院学报》2001年第4期335-336,共2页ACTA Academiae Medicinae Nantong

基　　金：国家自然科学基金资助项目 ( 30 0 70 2 5 5 );江省自然科学基金重点项目 ( BK992 0 1)

摘　　要：目的 :用荧光素逆行追踪试验了解人工组织神经移植物辅加神经再生素桥接缺损坐骨神经后 ,神经再生修复的情况。方法 :人工组织神经移植物辅加神经再生素桥接狗缺损的 30 m m坐骨神经手术后 6个月时 ,于原远端吻合口下 10 mm处注射快蓝 (FB) ;取脊髓及相应背根神经节 ,观察神经元被荧光素标记情况。结果 :脊髓及背根神经节中均出现数量不等的被 FB标记的神经元胞体。结论Objective: To explore the possibility of repair dog sciatic nerve 30mm gaps with artificial nerve graft adding Nerve Regeneration Factor (NRF) with fluorescence retrograde tracing.Methods: The sciatic nerve gaps 30mm of dogs were sutured with artificial nerve graft adding NRF. The fast blue was injected into the distal sciatic nerve after six months; the traversing spinal cord segment and the dorsal root ganglion were observed under fluorescence microscope. Results: The positive cells labeled fast blue were found in the spinal cord segment and the dorsal root ganglion.Conclusions: New nerve graft with NRF has been successful in the repaired sciatic nerve 30mm gap on dogs.

关 键 词：神经再生 人工组织神经移植物 坐骨神经缺损 荧光素逆行追踪试验 动物实验 

分 类 号：R322－33[医药卫生—人体解剖和组织胚胎学]