紫草素对人胎肾系膜细胞凋亡的影响  

Effect of Shikonin on Human Fetal Mesangial Cell Apoptosis

作　　者：李海涛[1] 李晓冬[1] 朱荃[1] 方泰惠[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029

出　　处：《药学进展》2001年第6期367-369,共3页Progress in Pharmaceutical Sciences

摘　　要：采用流式细胞仪和DNA含量测定方法检测细胞凋亡 ,观察紫草素对RPMI 16 40培养基及高糖培养的人肾小球系膜细胞凋亡的影响 ,探讨紫草素对肾小球硬化的治疗作用机制。结果表明 ,紫草素对人胎肾系细胞凋亡具抑制作用 ,尤可显著降低 80mmol/L葡萄糖诱导的高凋亡率 ,提示紫草素具有延缓肾小球硬化的作用。DNA electrophoresis and flowcytometry were used for assessing apoptosis. The effect of shikonin on human fetal glomerular mesangial cell apoptosis in RPMI 1640 and glucose-containing cultures was observed in order to explore the acting mechamism of shikonin on glomerulosclerosis. The results showed that shikonin significantly inhibited human fetal glomerular mesangial cell apoplosis, especially in glucose-adding culture. It may also slow the process of glomerulosclerosis.

关 键 词：紫草素 人胎肾细胞 细胞凋亡 肾小球硬化 动物实验 

分 类 号：R285[医药卫生—中药学]