人前列腺组织中雄激素受体亚型表达与基因克隆  

Androgen receptor isoforms expression and its novel cDNA cloning in human prostate

作　　者：夏术阶[1] 王延军[2] 唐孝达[1] 

机构地区：[1]上海市第一人民医院泌尿外科,200080 [2]山东省立医院中心实验室

出　　处：《中华医学杂志》2001年第24期1500-1502,共3页National Medical Journal of China

基　　金：中国博士后科学研究基金资助项目 ( 2 0 0 0 2 3 2 7)

摘　　要：目的　研究人前列腺组织中雄激素受体 (AR)亚型的表达及其基因学基础。方法　应用聚丙烯酰胺凝胶等电泳和分子生物学基因克隆技术 ,对 1例前例腺增生症 (BPH)患者手术切除的前列腺组织中的AR亚型蛋白表达与AR亚型基因进行了研究。结果　在该例患者增生良性前列腺组织中 ,发现有 3个AR亚型蛋白表达 ,并克隆出了 1条有 1913个核苷酸组成的AR亚型cDNA ,命名为PHAR 1。GenBank登记号为 :BankIT372 2 0 5AF32 42 43。PHAR 1与GenBank中已登记的AR序列比较 ,有 3段序列共 96 1个核苷酸相同 ,有 95 2个核苷酸组成的序列不同 ,显示了这条新的ARcDNA的同源性和特异性。结论　同一组织标本的 3个AR亚型蛋白表达并克隆 1条ARcDNA基因的结果 ,提示AR亚型蛋白可能源于同一基因的不同起始密码子的转录作用。AR亚型cDNA的克隆为研究AR亚型的调控与转录、AR亚型蛋白表达与前列腺增长 ,BPH与前列腺癌的发病机制、筛选阻断与激活AR亚型基因的药物 ,探讨治疗前列腺癌的新策略奠定了基础。Objective To investigate the androgen receptor isoforms expression and its novel cDNA cloning in prostate tissue. Methods The tissue from a case of benign prostatic hyperplasia(BPH)was analyzed by high resolution isoelectric focusing (IEF) technique and molecular biological technique of cDNA cloning was used to determine its AR isoforms expression and genes of AR isoforms. Results From the prostatic specimen, three types of AR isoforms were detected with pI values of 6.5, 6.0 and 5.3 respectively. A novel AR subtype cDNA cloning, named as PHAR 1 and including 1 913 nucleotides was isolated from the same BPH tissue. The GenBank accession number for the nucleotide sequence of this AR cDNA was BankIT372205 AF324243. The comparison between PHAR 1 and AR nucleotide sequence registered in GenBank showed that 961 nucleotides in three part of nucleotide sequence were the same, while the other 952 nucleotides were different. Conclusion These three AR isoforms may originate from the same gene. The results may be important for the study of BPH, or prostatic growth. It is also valuable in the field of pharmacotherapy for treatment of BPH and prostatic cancer. [

关 键 词：前列腺 雄激素受体亚型 基因克隆 前列腺癌 

分 类 号：R697[医药卫生—泌尿科学]