人类补体衰变加速因子对小鼠细胞保护作用的研究  

Protective effect of human decay accelerating factor on mouse cells

作　　者：赵玉千[1] 李路江[2] 付耀文[2] 吴长利[3] 

机构地区：[1]首都医科大学附属北京天坛医院泌尿外科,100050 [2]吉林大学中日联谊医院泌尿外科 [3]天津医科大学第二附属医院泌尿外科

出　　处：《中华医学杂志》2001年第24期1523-1525,共3页National Medical Journal of China

摘　　要：目的　观察人类补体衰变加速因子 (DAF)在小鼠细胞表达后 ,对小鼠细胞抗人血清补体破坏的保护作用。方法　用人DAFcDNA转染小鼠细胞使该基因在小鼠细胞中表达。再将此种小鼠细胞放入含正常人血清的培养基中孵育 ,观察小鼠细胞是否被破坏。结果　转染人DAFcDNA并表达人DAF的小鼠细胞 ,在含人正常血清的培养基中孵育 48h后仍能存活并可以传代 ,而对照组的小鼠细胞孵育后在 1～ 4h内迅速死亡。结论　人类的DAF可以保护小鼠细胞不受补体的破坏。Objective To observe the protective effect of human decay accelerating factor (DAF) expressed in mouse myeloma cells against the injury of human serum complement. Methods Mouse myeloma cells were transfected with human DAF cDNA and then put into normal human serum culture to obsetrve if they were injured. Results The mouse cells containing human DAF cDNA and expressing human DAF survived for more than 48 hours and passed on from generation to generation in the culture medium containing human normal serum. However, the control mouse cells died in 1～4 hours. Conclusion Human DAF protects mouse cells from injuring by human complement.

关 键 词：补体 转基因 移植物排斥 异种移植 动物实验 器官移植 DAF 

分 类 号：R392.1[医药卫生—免疫学]