神经节苷脂GM_1对缺血缺氧后脑中HSP_(70)表达的影响  

Effects of GM_1 on the expression of HSP_(70) in the neonatal rats following hypoxia-ischemia

作　　者：胡志兵[1] 陆雪芬[1] 郑德枢[1] 邓平[2] 

机构地区：[1]广州医学院神经科学研究所 [2]广州市第十二人民医院神经内科,硕士研究生510620

出　　处：《卒中与神经疾病》2001年第5期278-281,共4页Stroke and Nervous Diseases

摘　　要：目的　研究神经节苷脂GM1对新生鼠缺血缺氧性脑损伤的保护作用及HSP70 表达的影响。方法　建立新生鼠缺氧缺血性脑病动物模型 ,应用组织化学和免疫组织化学法观察缺血缺氧后 3h、6h、1d、3d、7d、14d脑组织的病理变化及HSP70 的表达 ,以及GM1给药后的变化。结果　单纯缺血缺氧组 ,在缺血缺氧后 3h缺血侧皮层、海马CA3 区、纹状体开始有少量HSP70 表达 ,2 4h达高峰 ,14d未见HSP70 表达 ;GM1给药组 ,脑组织损伤明显减轻 ,6h才开始有少量HSP70 表达 ,7d未见有表达。结论　GM1对新生鼠缺血缺氧性脑损伤具有明显的保护作用 ;HSP70 的诱导表达是缺血缺氧性脑损伤敏感而可靠的指标 ,GM1可抑制这种表达。Objective To study the neuroprotective effects of ganglioside GM 1 and the effects of ganglioside GM 1 on the HSP 70 expression in the neonatal rats after hypoxia ischemia (HI).Methods In the animal model of neonatal rat with hypoxic ischemic encephalopathy (HIE), using histo chemistry and immunohisto chemistry method, we observed pathology and HSP 70 expression in the brain at the point of 3 h, 6 h, 24 h, 3 d, 7 d, 14 d after HI and the changes after GM 1 was administered. We also investigated the change of HSP 70 expression.Results The damages in the GM 1 group was less than that in the HI group, HSP 70 expression was observed in a small amount on the ipsilateral cortex, hippocumpus CA 3 and striutum at 3 h, the highest at 24 h and no change at 14 d; but in the GM 1 group of HSP 70 expressions did not begin until 6 h after HI, were milder than in the HI group during 6 h～3 d after episode of HI,no change at 7 d.Conclusions GM 1 has protective effects on neonatal rat with HIE. The induced expression of HSP 70 is a useful index in the brain injury, GM 1 can inhibite the expression of HSP 70 induced by hypoxia ischemia.

关 键 词：缺血缺氧性脑病 新生鼠 热休克蛋白70 神经节苷脂GM1 

分 类 号：R722.12[医药卫生—儿科]