P38激酶介导胆红素诱导的SHSY5Y细胞凋亡  

P38 MAP kinase mediates bilirubin-induced apoptosis in SHSY5Y cells

作　　者：韩张[1] 胡萍[2] 倪道凤[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉科,北京100730 [2]中国医学科学院中国协和医科大学基础医学研究所药理室,北京100005

出　　处：《基础医学与临床》2002年第1期45-46,共2页Basic and Clinical Medicine

摘　　要：观察P38激酶在胆红素诱导神经细胞凋亡中的作用 ,探讨参与此过程的信号转导通路。将胆红素 (2× 1 0 -2g L)直接作用于体外培养的人神经母细胞瘤细胞系SHSY5Y ,利用Hochest332 58染色在荧光显微镜下观察细胞核的形态是否发生凋亡样改变 ;用P38激酶抑制剂SB2 0 3580预处理细胞后 ,在倒置光显微镜下观察胆红素作用不同时间细胞形态的变化及存活情况 ;利用流式细胞仪检测细胞凋亡率的改变。结果显示SHSY5Y细胞经胆红素作用后细胞核出现典型的凋亡样改变 ;细胞经SB2 0 3580预处理 1h ,胆红素作用 3h后凋亡率为 (1 2 1± 2 4) % ,对照组为(1 9 4± 2 7) % ;4h后凋亡率为 (39 3± 4 8) % ,对照组为 (66 2± 5 2 ) % ,差异有显著性意义 (P <0 0 1 )。To study the role of P38 MAP kinase in the process of bilirubin induced apoptosis in Human neuroblastoma cell line SHSY5Y cells. After exposure to bilirubin the morphological changes of nucleus of SHSY5Y cells were examined by Hochest33258 staining. Pretreated with P38 MAP kinase inhibitor SB203580 SHSY5Y cells were exposed to bilirubin, then morphologic change was examined by phase contrast microscope; Apoptotic cells were measured by flow cytometry. The results showed the typical apoptotic nuclear changes that emerged after exposing to 2×10 -2 g/L bilirubin for 6h. Pretreated with 50μmol/L SB203580 for 1 h , apoptotic cells significantly decreased from (19.4±2.7)% to (12.1±2.4)% after exposing to 2×10 -2 g/L bilirubin for 3h, and (66.2±5.2)% to (39.3±4.8)% after 4h ( P <0.01). The results suggested that P38 MAP kinase mediated bilirubin induced apoptosis in SHSY5Y cells.

关 键 词：胆红素 P38激酶 凋亡 人神经母细胞瘤细胞系 

分 类 号：R722.17[医药卫生—儿科]