可溶性白细胞介素2受体在急性移植物抗宿主病诊断中的意义  

Implication of sIL-2R in the diagnosis of acute graft-versus-host disease

作　　者：居小萍[1] 王健民[1] 章卫平[1] 童书鹏[1] 许燕群[1] 龚胜蓝[1] 

机构地区：[1]第二军医大学附属长海医院血液科,上海200433

出　　处：《中华器官移植杂志》2002年第1期42-44,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金资助项目 (3 9870 710 );上海市卫生系统"百人计划"资助项目 (98BR0 2 9)

摘　　要：目的　研究造血干细胞移植后细胞因子及其受体水平的变化与急性移植物抗宿主病(aGVHD)的关系 ,以探讨预测aGVHD的临床指标。方法　 30例血液病患者行异基因造血干细胞移植 ,采用双夹心酶联免疫吸附法 (ELISA)动态监测血清中可溶性白细胞介素 2受体 (sIL 2R)、白细胞介素 6、白细胞介素 8、干扰素及肿瘤坏死因子水平的变化。结果　 30例患者均获得造血重建 ,10例发生Ⅰ度GVHD ,5例发生Ⅲ～Ⅳ度GVHD ;sIL 2R在发生aGVHD组于移植后第 2周上升 ,第 3周达峰值 ,其升高早于aGVHD症状的出现 ,与疾病的严重程度显著相关 ,随症状的缓解而下降 ;其它细胞因子水平的变化不显著 ,与aGVHD的发生无明显关系。结论　造血干细胞移植后aGVHD的发生伴随某些细胞因子水平的变化 ,sIL 2R在aGVHD症状出现前明显上升 。Objectives To investigate the change of cytokines and their receptors after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and their relation with human acute graft-versus-host disease (aGVHD). Method Serum concentrations of sIL-2R, IL-6, IL-8, TNF-alpha, IFN-beta were measured periordically by using enzymed-linked immunosorbent assay (ELISA) in 30 patients undergoing allo-HSCT.Results All patients achieved engraftment. Ten patients developed grade ⅠGVHD, 5 grade Ⅲ-Ⅳ GVHD. Patients with aGVHD had significantly higher sIL-2R levels than those without aGVHD. The increase of sIL-2R level in serum preceded the clinical signs of aGVHD. The maximum levels of sIL-2R were correlated significantly with the severity of aGVHD.Conclusion Detection of serum cytokines after allo-HSCT might provide a predictive marker for aGVHD.

关 键 词：移植物抗宿主病 细胞活素类 诊断 SIL-2R 

分 类 号：R617[医药卫生—外科学]