RP-HPLC测定小鼠肿瘤组织中洛莫司汀的含量  

Determination of the Content of Lomustine in Mouse Tumor Tissue by RP-HPLC

作　　者：孙勇 张理星 周海英[2] 张其清[2] 吴晓渔 

机构地区：[1]山东鲁南制药股份有限公司,临沂市276006 [2]中国协和医科大学,中国医学科学院生物医学工程研究所,天津市300192 [3]加拿大多伦多药学院

出　　处：《中国药房》2001年第9期518-519,共2页China Pharmacy

基　　金：1996年度国家杰出青年科学基金资助项目(59625306)

摘　　要：目的：以反相高效液相外标法测定小鼠肿瘤组织内洛莫司汀的含量。方法：应用乙腈－水（５４：４６）为流动相，在Ｃ１８（５ｕｍ）色谱柱上进行测定。结果：洛莫司汀的检测线性范围为０．９８～１５，６８ｕｇ／ｍｌ，ｒ＝０．９ ９９９。回收率为６８．６４％（ｎ＝９）ＲＳＤ为０．３２％。结论：本法简便、快速、灵敏、重现性好。OBJECTIVE: To determine the content of lomustine in mouse tumor tissue. METHODS: Lomustine was separated and determined on a reverse-phase C18 5um column with a mobile phase of acetonitrile-H2O(54 : 46), detected at 254nm and no internal standard. RESULTS: The ca[ibration curve was 1ineaI(r = 0. 9 999) within the range of 0. 98- 15. 68ug/ml for lomus tine. The recovery ratio of lomustine was 68. 64% (n = 9). The relative standard deviation(RSD) was 0. 32%. CONCLUSION: This method is simple and accurate

关 键 词：洛莫司汀 反相高效液相色谱法 肿瘤组织 

分 类 号：R979.1[医药卫生—药品] R965.1[医药卫生—药学]