感染大链壶菌后致倦库蚊幼虫血淋巴细胞的观察  

Observation on Hemocytes of Culex pipiens quinquefasciatus Larvae Infected by Lagenidium giganteum

作　　者：莫非[1] 包怀恩[2] 

机构地区：[1]贵阳医学院检验系,贵阳550004 [2]贵阳医学院寄生虫学教研室,贵阳550004

出　　处：《中国寄生虫学与寄生虫病杂志》2001年第6期330-332,F003,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金 (No .3 9460 0 3 4)~~

摘　　要：目的　探讨致倦库蚊幼虫被大链壶菌感染后可能的免疫防御反应。方法　采用蚊血淋巴涂片 ,姬氏染色光镜检查法结合相差显微镜技术 ,对致倦库蚊幼虫的血淋巴细胞进行观察计数和显微摄影分析。结果　①大链壶菌感染后随感染时间的延长蚊幼单位体积血淋巴细胞数出现“增加、急剧增加、然后逐渐减少”的变化特点。②在感染后 4 8和 72h浆血胞和粒血胞比例明显增加 ,原血胞比例下降 ;在感染后 96及 12 0h ,原血胞比例相对增加 ,珠血胞和类绛血胞所占比例越来越大 ,浆血胞与粒血胞比例相对减少。③感染大链壶菌后 ,浆血胞发生空泡、细胞变形等变化。结论　感染大链壶菌后致倦库蚊幼虫出现血淋巴细胞数量、分类及形态的变化 ,显示大链壶菌在蚊体内诱发了一定程度的免疫防御反应 。Objective To explore the possible defensive immunity of Culex pipiens quinquefasciatus larvae after being infected by Lagenidium giganteum . Methods To count and analyze morphologically hemocytes of the mosquito larvae with Giemsa's stain and by both phase contrast microscope and ordinary microscope. Results ① Following the prolonged duration of infection, the number of hemocytes featured as increase, intense increase and gradual decrease. ② 48 and 72 h after infection the proportion of plasmatocytes and granulocytes increased significantly, but that of prohemocytes showed a relatively slow increase, while 96 and 120 h after infection the proportion of spherulocytes and oenocytoids increased gradually and that of plasmatocyte and granulocytes showed a slow decrease. ③ After L. giganteum infection, vacuolation and morpholysis were found in plasmatocytes. Conclusion The changes of hemocytes in amount,classification and morphology after infection revealed that Lagenidium giganteum can induce some defensive immune reactions in the larvae of Culex pipiens quinquefasciatus possibly due to a release of antigens or toxic substances by the fungus.

关 键 词：致倦库蚊幼虫 大链壶菌 血淋巴细胞 防御性免疫 生物灭蚊剂 

分 类 号：R979.8[医药卫生—药品]