斑点金免疫渗滤法在检测囊虫抗体中的应用  

Application of Dot Immunogold Filtration Assay in Antibody Detection for Cysticercosis

作　　者：刘志平[1] 李浩[1] 朱兰芸 刘森[1] 薛海筹[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所 [2]上海市博爱医院,上海200031

出　　处：《中国寄生虫学与寄生虫病杂志》2001年第6期354-356,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：科技部重点资助项目 (No .96 92 0 3 7 0 6)~~

摘　　要：目的　将斑点金免疫渗滤法用于检测囊虫抗体 ,以制备囊虫病诊断试剂盒。方法　经透析的猪囊尾蚴囊液作为检验抗原 ,对 71份囊虫病患者、90份正常人和 2 0份其他病患者血清进行了斑点金免疫渗滤法检测并与酶联免疫吸附法比较。结果　斑点金免疫渗滤法的敏感性为 90 .1% (6 4 / 71) ,特异性为 95 .6 % (86 / 90 ) ,其他病患者血清反应中仅 1例脑肿瘤患者血清阳性 ,其余为阴性。斑点金免疫渗滤法和酶联免疫吸附法的符合率为94 .4 % (15 2 / 16 1)。Objective To detect serum antibody of cysticercosis by dot immunogold filtration assay for establishing a diagnostic kit for cysticercosis patients. Methods Cyst fluid of cysticercus of Taenia solium after dialysis was used as diagnostic antigen in dot immunogold filtration assay and enzyme linked immunosorbent assay to detect the serum antibody in patients with cysticercosis. Samples to be detected included 71 sera from patients with cysticercosis, 90 sera from healthy people, 20 sera from patients with other parasitic infections or brain tumor. Results The sensitivity and specificity of dot immunogold filtration assay were 90.1%(64/71)and 95.6%(86/90), respectively. No positive reaction was recorded in cases with other diseases except one serum from a patient with brain tumor. The coincidence rate between dot immunogold filtration assay and enzyme linked immunosorbent assay was 94.4%(152/161). Conclusion Dot immunogold filtration assay showed promising result for the diagnosis of cysticercosis.

关 键 词：斑点金免疫渗滤法 DIGFA 猪囊尾蚴囊液 酶联免疫吸附法 ELISA 囊尾蚴病 

分 类 号：R532.32[医药卫生—内科学] R446.61[医药卫生—临床医学]