冠心病患者载脂蛋白CII微卫星DNA多态性与血清脂质水平的关系  

Genetic polymorphism analysis of apolipoprotein CII microsatellite DNA (TG) n (AG) m in patients with coronary heart disease

作　　者：高纯[1] 周新[1] 尹志农[1] 朱名安[1] 

机构地区：[1]武汉大学第二临床学院医学检验系

出　　处：《中华医学杂志》2001年第23期1436-1438,共3页National Medical Journal of China

基　　金：湖北省科技攻关重点课题基金资助 ( 97j138)

摘　　要：目的　研究载脂蛋白CII(apoCII)微卫星DNA多态性与冠状动脉粥样硬化性心脏病 (冠心病 )及其血脂水平的关系。方法　采用聚合酶链反应结合高压变性聚丙烯酰胺凝胶电泳 ,检测了2 31例健康人及 10 8例冠心病患者的apoCII微卫星DNA (TG)n(AG)m基因型。结果　冠心病组与对照组等位基因频率差异具有显著性意义 (χ2 =19 93,P <0 0 5 ) ,冠心病组等位基因 17频率 (0 148)明显高于正常对照组 (0 0 6 1,χ2 =13 98,P <0 0 1) ,17/ 2 9型、17/ 30型较其他基因型TG高、HDL C低 ,具有显著性差异 (P <0 0 5 )。结论　apoCII等位基因 17可能为汉族人群冠心病的一个独立危险因素。Objective To investigate the relationship between the polymorphism of apolipoprotein CII (apoCII) microsatellite DNA (TG)n(AG)m and coronary heart disease (CHD). Methods The apoCII microsatellite DNA (TG) n(AG)m genotypes of 108 patients with CHD and 231 healthy persons were detected by using polymerase chain reaction (PCR) and high voltage denatured polyacrylamid gels electrophoresis. Results The allele frequency distribution was significantly different between controls and patients with CHD (χ 2=19.93, P < 0.05). The allele 17 frequency in CHD group was remarkably higher than that in controls (χ 2=13.98, P <0.01). The concentrations of TG and HDL C among different genotype groups were significantly different. The levels of TG in 17/29 group and 17/30 group were higher than those in other groups ( P <0.05), while the concentration of HDL C was lower ( P <0.05). Conclusion The allele 17 of apoCII may be an independent risk factor for CHD in Han population.

关 键 词：载脂蛋白CⅡ 微卫星DNA 基因多态性 冠心病 血脂 脂蛋白 

分 类 号：R446.9[医药卫生—诊断学]