镉对Ⅱ型糖尿病小鼠金属硫蛋白基因表达的诱导与镉肾脏毒性之关系  

Ability of Induced Expression of Metallothionein Genes Related to the Susceptibility of Type-2 Diabetic Mice to Nephrotoxicity of Cadmium-metallothionein

作　　者：逄兵[1] 金泰廙[1] 周袁芬[1] 蒋学之[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生教研室,上海200032

出　　处：《劳动医学》2001年第4期196-198,共3页

基　　金：瑞典Sida基金 (Sida -Umea99)资助项目

摘　　要：[目的 ]探讨金属硫蛋白基因诱导表达能力与Ⅱ型糖尿病小鼠 (ob/ob小鼠 )对镉金属硫蛋白肾脏毒性易感性之间的关系。 [方法 ]以 β actin为内参照 ,采用半定量RT PCR方法检测在相同肾皮质镉水平条件下 ,镉对ob/ob小鼠和瘦型小鼠 (正常小鼠 )肾皮质MT1和MT2基因的诱导表达。 [结果 ]镉对ob/ob小鼠肾皮质MT1和MT2基因诱导表达的能力过低 ,而镉对瘦型小鼠MT1及MT2基因具有明显的诱导能力。 [结论The aim of this study was to explore the ability of induced expression of metallothionein genes related to the susceptibility of type 2 diabetic mice (ob/ob mice) to cadmium metallothionein(CdMT) induced nephrotoxicity. With semi quantitative RT PCR,gene expressions of MT1 and MT2 induced by the same cadmium level in the renal cortex of type 2 diabetic mice and their lean litter mates (normal mice) were studied. Gene expressions of MT1 and MT2 in the renal cortex of ob/ob mice could not be induced obviously by CdMT. However they could be induced significantly in that of normal mice. [Conclusion] The authors concluded that there were some relationships between the possible mechanisms of the susceptibility of type 2 diabetic mice to CdMT induced nephrotoxicity and the lower ability of induced expressions of MT1 and MT2 genes in the renal cortex.

关 键 词：镉中毒 镉金属硫蛋白 Ⅱ型糖尿病 肾脏毒性 金属硫蛋白基因 诱导表达 

分 类 号：R135.1[医药卫生—劳动卫生]