IFN-γ、IL-1诱导人脑膜成纤维细胞IDO抗弓形虫增殖  

IFN-γ and IL-1 Block Toxoplasma gondii Growth by Inducing Indoleamine 2,3-Dioxygenase in Human Meninx Fibroblast

作　　者：资捷[1] 朱家勇[1] 

机构地区：[1]广东医学院寄生虫学教研室,广东湛江524023

出　　处：《实用寄生虫病杂志》2001年第4期149-151,共3页Journal of Practical Parasitic Diseases

基　　金：广东省高教厅基金资助项目 ( No.992 5 )

摘　　要：目的探讨 IFN-γ、IL-1诱导人脑膜成纤维细胞 ( Human meninx fibroblast,HMF)抗弓形虫感染的机制。方法采用 3 H-尿嘧啶 ( 3 H-U )特异标记的弓形虫在 HMF内的增殖实验 ,以及通过反相高效液相色谱仪检测吲哚胺 2 ,3-双加氧酶 ( indoleamine 2 ,3-dioxygenase,IDO)的活性。结果 IFN-γ、IL-1单独或协同都可诱导 HMF产生 IDO,降解胞内必需氨基酸色氨酸 ,弓形虫合成蛋白质时 ,由于发生“色氨酸饥饿”而被抑制生长 ,加入外源性色氨酸可逆转 IFN-γ、IL-1对弓形虫的抑制作用。结论诱导 HMF产生 IDO降解胞内色氨酸是 IFN-γ、IL -1抗弓形虫感染的机制。Objective In vitro cultured human meninx fibroblasts (HMF) were treated with IFN γ or/and IL 1 and the antitoxoplasma mechanism of IFN γ or/and IL 1 was studied. Method The ability to restrict the growth of Toxoplasma gondii was evaluated by 3 H U incorporation assay. Indoleamine 2,3 dioxygenase (IDO) activity was determined with high performance liquid chromatography (HPLC). Results IDO was induced by IFN γ or/and IL 1 in HMF, which could degrade the intracellular tryptophan. T.gondii growth was then restricted because of hypotryptophan during the process of protein synthesis. Addition of exogenous tryptophan to the culture medium blocked the antiparasitic effect. Conclusion IFN γ or/and IL 1 was involved in the defense against toxoplasmosis by inducing IDO activity to degrade the intracellular tryptophan as an antitoxoplasma mechanism in HMF.

关 键 词：弓形体 吲哚胺2 3-双加氧酶 人脑膜成纤维细胞 IFN-Γ IL-1 弓形体脑炎 

分 类 号：R382.5[医药卫生—医学寄生虫学] R531.8[医药卫生—基础医学]