和胃消痞胶囊对大鼠胃肠肌间神经丛一氧化氮合酶的影响  

Effect of Heweixiaopi Capsule on NOS in Intermuscular Nerve Plexus of Stomaches and Intestines of Rats

作　　者：夏立营[1] 葛文津[1] 李明喜[1] 

机构地区：[1]中国中医研究院西苑医院消化科,北京100091

出　　处：《中国中西医结合消化杂志》2001年第5期259-260,共2页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家科委九五重点科技攻关项目 (No.96 - C0 3- 0 1- 0 7)

摘　　要：目的 :研究和胃消痞胶囊对大鼠胃肠肌间神经丛一氧化氮合酶 (NOS)的影响 ,探讨其作用机制。方法 :采用隔日禁食的方法制作大鼠胃肠运动功能紊乱模型 ,以辅酶 黄递酶组化染色法及消化道铺片技术显示大鼠胃及上段小肠纵肌层 NOS阳性神经。结果 :胃窦 NOS阳性神经元灰度值模型组 (模型组 )低于正常对照组 ;和胃消痞胶囊大、小剂量组低于对照组 ,但高于模型组 ;中剂量组高于模型组及对照组 ;吗丁啉组低于模型组及对照组。胃底模型组及和胃消痞胶囊大、中剂量组均低于对照组 ;小剂量组及吗丁啉组高于对照组。上段模型组及和胃消痞胶囊小剂量组与对照组接近 ,大、中剂量组及吗丁啉组显著高于对照组。结论 :和胃消痞胶囊可预防模型鼠胃窦动力紊乱 ;大剂量增加胃底 NO合成 ,小剂量使胃底 NO合成减少 ,对胃底容受性舒张功能有双向调节作用 ;大、中剂量可减少上段小肠 NO合成 。Objective:To study the mechanism of Heweixiaopi capsule on NOS in intermuscular nerve plexus of stomaches and intestines of rats.Methods:To make models of gastrointestinal dysfunction by fasting rats every other day.To show NOS positive nerve in longitudinal muscle layer of stomach and superior segmental intestine by the method of histochemistry stain with NADPH Ⅱ and diaphorase.Results: In gastric antrum,the gray value of Heweixiaopi big and middle dosage groups were lower than the normal control group(P< 0.01 ),and the gray value of the big dosage group was also lower than the model group(P< 0.05 );the gray value of the small dosage group was higher than the model group(P< 0.01 ).In superior segmental intestine,the gray value of the middle dosage group was higher than the normal control group and the model group(P< 0.01 ).Conclusion:Heweixiaopi capsule can prevent the disorder of gastric antrum of model rats;can regulate the relax function of gastric fundus in bidirection;the big and middle dosage can improve the peristaltic function of intestine.

关 键 词：胃肠活动 一氧化氮合酶 和胃消痞胶囊 还原型辅酶Ⅱ 黄递酶 

分 类 号：R285.5[医药卫生—中药学]