康胃方对沙土鼠幽门螺杆菌的根除及胃粘膜保护作用  

Effects of Kangwei Fang for Eradicating Helicobacter pylori and Protecting Gastric Mucosa in Mongolian Gerbil

作　　者：赵子厚[1] 陈飞松[1] 张锁雅[1] 梁代英[1] 任蜀兵[1] 刘晋生[1] 李春梅[1] 

机构地区：[1]北京市中医研究所,北京100010

出　　处：《中国中西医结合消化杂志》2001年第5期261-263,267,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：北京市自然科学基金资助项目 (No. 7972 0 0 7)

摘　　要：目的 :观察康胃方对沙土鼠幽门螺杆菌 (Hp)根除及胃粘膜细胞保护作用。方法 :蒙古沙土鼠 42只 ,随机分为病理模型组、三联治疗组及康胃方高、低剂量组 ,每日灌胃给予 Hp菌液 ,共 3次。用脲呼吸试验 (UBT)确认 Hp感染情况并接受相应的治疗药物。另 12只动物未予感染。治疗后第 4周处死动物 ,测定胃粘膜活性氧 (RO)、一氧化氮 (NO)、诱生型一氧化氮合成酶 (i NOS)、Mn SOD及 NPSH。结果 :Hp感染各组动物 ,UBT与胃粘膜所含 RO、NO、i NOS和 Mn SOD明显升高 ,但 NPSH显著下降 (P <0 .0 1)。经抗感染治疗后 UBT值降低 ,其他指标明显改善(P <0 .0 1)。结论 :康胃方对蒙古沙土鼠感染 Hp有明显的根除作用 ,并通过改善感染动物胃粘膜某些生化成分的含量或活性 ,发挥其细胞保护作用。Objective:To infect Mongolian gerbil with Helicobacter pylori and study the effects of Kangwei Fang for eradicating Hp and protecting gastric mucosa.Methods:42 Mongolian gerbil were randomly divided into four groups, model and triple therapy and Kangwei Fang high/low doses group. Animals were inoculation orally Hp strain (Sydney strain 1). 4 weeks after inoculation, the animals from each group received treatment respectively. Another 12 uninfected Monglian gerbil served as control.The evidence for Hp infected was assessed by 3 times of Urea Breath Test(UBT). At the 4th week the animals were sacrificed and observed the amount or activity of reactive oxygen, nitric oxide, inducible nitric oxide synthetase, superoxide dismutase and NPSH in homogenate of gastric mucosa. Results: UBT values were higher with Hp infected animals compared with uninfected animals (P< 0.01 ), but it showed markedly decrease of values after antimicrobial therapy (P< 0.01 ), the amount and activity of RO, NO, iNOS and MnSOD in gastric mucosa significantly increased in Hp treated animals, the while NPSH were found fall (P< 0.01 ). After treatment with Kangwei Fang, the above mentioned indexes were obviously improved. Conclusion:These results suggest that Kangwei Fang possessed obviously the eradication of Hp and had a protective actions against the changes of the certain biochemical substances of the gastric mucosa in Mongolian gerbil with Hp infected.

关 键 词：康胃方 螺杆菌感染 胃粘膜 

分 类 号：R285.5[医药卫生—中药学]