XW630对大鼠成骨细胞TGF-β_1和Ⅰ型胶原基因表达调控的研究  

Regulation of XW630 on TGF-β_1 and Collagen Ⅰ Gene Expression in Osteoblasts

作　　者：费伟[1] 王大章[2] 郑虎[3] 翁玲玲[3] 邓力[3] 

机构地区：[1]四川省人民医院口腔颌面外科 [2]四川大学华西口腔医学院口腔颌面外科学教研室 [3]四川大学华西药学院药物化学研究室

出　　处：《华西口腔医学杂志》2001年第6期383-391,共9页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (编号 3 943 0 12 0 )

摘　　要：目的 :探讨抗骨质疏松新药XW6 30促进成骨作用的机理。方法 :提取并标记制备TGF_β1和ColⅠ基因的cDNA探针 ,将 3种不同药物作用于大鼠成骨细胞 2 4、72h后 ,提取细胞总RNA ,并与TGF_β1和ColⅠ基因的cDNA探针进行狭缝杂交。结果 :XW6 30具有促进大鼠成骨细胞中TGF_β1和ColⅠmRNA表达的作用 ,在相同浓度 (10 -6mol/L)下 ,其作用优于四环素加哌嗪雌酮和单纯雌酚酮 (P <0 0 5 )。结论 :XW6Objective:The purpose of this part of study was to investigate the mechanism of a new anti_osteoporotic drug, XW630 on promoting osteogenic activity.Methods:Total RNA were isolated from osteoblasts of the mice after the reaction with XW630 in 24 and 72 hours, that were hybridized with the extracted and digoxigenin_labeled TGF_β\-1 and the collagenⅠ cDNA probes.Results:XW630 significantly promoted TGF_β\-1 and collagenⅠ gene expression in osteoblasts, and this drug was superior than other two kinds of estrogen in promoting osteogenic activity (P<0.05).Conclusion:XW630 promote osteogenic activity of osteoblasts, probably by enhancing the mRNA expression of TGF_β\-1 and collagenⅠ.\;

关 键 词：成骨细胞 转化生长因子Β1 I型胶原 基因表达 抗骨质疏松药 XW630 大鼠 

分 类 号：R965[医药卫生—药理学]