山羊乳中上皮粘蛋白MUC1的遗传多态性  

Genetic Polymorphism of Epithelial Mucin, MUC1,in Milk of Goats

作　　者：彭先文 钟光辉[2] 郑玉才[2] 毛永江[2] 王永[2] 江明峰[2] 

机构地区：[1]湖北省畜牧兽医研究所,湖北武汉430209 [2]西南民族学院动物科技系,四川成都610041

出　　处：《Zoological Research》2002年第1期15-18,共4页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 39870 6 0 7);四川省科委资助课题

摘　　要：采用SDS PAGE方法研究了波尔山羊、成都麻羊、安哥拉山羊×藏山羊F1、建昌黑山羊、安哥拉山羊×建昌黑山羊F1乳MUC1的生化遗传特性。结果表明 :山羊乳MUC1呈现出多态性 ,表现为 1条或 2条迁移率不同的区带。SDS PAGE分析发现 4种分子量的MUC1区带 ,即A、B、C和D ,分子量分别为 2 6 4、 2 41、 2 31和 2 2 0kDa ,大于牦牛和荷斯坦牛乳中的MUC1。基因型与山羊品种有关 ,在波尔山羊中最丰富 ,有 10种基因型 ,基因杂合度为 0 72 72 ;建昌黑山羊有 3种基因型 ,基因杂合度为 0 495 0 ;而在成都麻羊中仅发现CC基因型。经适合性检验 ,波尔山羊、成都麻羊、建昌黑山羊乳MUC1基因座基因型分布符合哈代The epithelial mucin,MUC1,was assayed using the SDS-PAGE method in individual milk samples from five populations,Boer goat,Chengdu Ma goat,Angora goat×Tibetan goat F 1,Jianchang black goat and Angora goat×Jianchang black goat F 1.Polymorphism is found in these goats' milk MUC1 with one or two bands showing the variable mobility and being stained the equal intensity.The bands present four polymorphic forms,i.e.A,B,C and D with molecular weights 264,241,231 and 220?kDa respectively,which are higher than those in Yak and Holstein cattle previously reported.There are differences in MUC1 genotype among the goat breeds:Boer goat exhibits ten genotypes and its gene heterozygosity is 0.7272,Jianchang black goat exhibits three genotypes and its gene heterozygosity is 0.4950,however Chengdu Ma goat only exhibits one CC genotype.Genotype distribution of milk MUC1 in Boer goat,Chengdu Ma goat and Jianchang black goat is in accordance with Hardy-Weinberg law.

关 键 词：山羊遗传乳 MUC1 多态性 上皮粘蛋白 

分 类 号：S827.1[农业科学—畜牧学] Q592.6[农业科学—畜牧兽医]