纤溶酶原激活因子与猕猴精子运动力的关系及其在附睾和附性腺中的表达调节  

Possible Involvement of Plasminogen Activator in the Acquirement of Sperm Progressive Motility and the Regulation of PA and PAI-1 mRNA Expressions in Epididymis,Prostate and Seminal Vesical in Rhesus Monkey

作　　者：郑萍[1] 邹如金[1] 刘以训[2] 

机构地区：[1]中国科学院昆明动物研究所生殖生物学实验室,云南昆明650223 [2]中国科学院动物研究所生殖生物学国家重点实验室,北京100080

出　　处：《Zoological Research》2002年第1期19-24,共6页动物学研究（英文）

基　　金：国家重点基础研究与发展规划项目 (G19990 5 5 90 1);中国科学院知识创新工程重要方向项目;国家自然科学基金资助项目 ( 39870 36 8)

摘　　要：用 11酸睾酮诱导猕猴少精子症和弱精子症及单侧隐睾手术诱导单侧少精子症和弱精子症模型 ,观察对附睾头、附睾体、附睾尾、前列腺和精囊组织型PA (tPA)、尿激酶型PA (uPA)及抑制因子 - 1(PAI 1)mRNA表达的影响。原位杂交的结果表明 11酸睾酮诱导少精子症和弱精子症 ,tPAmRNA的表达在附睾头、精囊及前列腺减少 ,而在附睾体升高 ,附睾尾表达基本无变化 ;uPAmRNA的表达在附睾头、附睾体、前列腺减少 ,而在精囊升高 ,附睾尾表达基本无变化 ;PAI 1mRNA的表达在附睾头、附睾体、精囊下降 ,而在前列腺升高 ,附睾尾表达无显著变化。单侧隐睾手术不影响tPA、uPA和PAI 1mRNA的表达。这些结果提示附睾头和附睾体分泌的uPA可能与精子前向运动能力的获得相关。tPA、uPA和PAI 1mRNA在猕猴附睾头部和体部、前列腺和精囊中的表达可能受睾酮的调节 ,但不受睾丸分泌因子及温度的影响 ,且在不同部位睾酮的调节具不同的特征 ,而附睾尾tPA、uPA和PAIRhesus monkeys were injected 11-T or were performed with unilateral cryptochidism to induce decrease of sperm count and motility.The expressions of tPA,uPA and PAI-1 mRNA in the normal and treated caput,corpus and cauda epididymis,prostate gland and seminal vesicle were examined by in situ hybridization.Results showed that after 9 weeks of 11-T injection,tPA mRNA expression increased in corpus,decreased in caput,seminal vesicle and prostate,while kept consistant in cauda;uPA mRNA expression increased in seminal vesical,decreased in caput,corpus and prostate,but remained unchanged in cauda;PAI-1 mRNA expression increased in prostate,decreased in caput,corpus and seminal vesicle,and no significant change was found in cauda.Unilateral cryptorchidism did not markedly alter the expressions for tPA,uPA and PAI-1 mRNA.These results suggest that ①uPA produced by caput and corpus epididymis may be involved in the acquirement of sperm progressive motility;②testosterone,but not other factors from testis or temperature regulate the tPA,uPA and PAI-1 mRNA expressions in caput,corpus epididymis,prostate and seminal vesical in a region-specific manner.However,the expressions for tPA,uPA and PAI-1 mRNA in cauda epididymis are regulated by none of the above factors.

关 键 词：猕猴 纤溶酶原激活因子 附睾 精子前向运动 PA 表达调控 附性腺 

分 类 号：Q959.848[生物学—动物学] Q492.4