长白蝮蛇类凝血酶基因(Ussurase)的克隆及分析(英文)  

Cloning and analysis of cDNA for thrombin-like enzyme,ussurase from Agkistrodon halys ussuriensis snake venom

作　　者：杜道林[1] 吴文芳[1] 吕国安[1] 徐梅[1] 邓晓东[2] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]热带作物生物技术国家重点实验室,海南海口571101

出　　处：《海南师范学院学报》2001年第3期85-93,共9页

基　　金：中国科学院"九五"重点项目(KZ-952-SI-226)

摘　　要：从长白蝮蛇（Agkistrodon halys Ussuriensis）毒腺中抽提总RNA,采用RT-PCR扩增其类凝血酶基因,经全序列测定,类凝血酶基因ussurase全长为699个核苷酸,即编码233个氨基酸;根据同源性,推测它的活性中心为His40,Asp85和Ser179;二硫键为Cys7-Cys138,Cys25-Cys41,Cys73-Cys231,Cys117-Cys185,Cys149-Cys164和Cys175-Cys200。该蛇毒类凝血酶cDNA序列及推导的氨基酸序列均为首次报道。Total RNA was extracted from the venom gland of the Agkistrodon halys Ussuriensis snake. The thrombin - like enzyme gene was amplified by RT -PCR, cloned, and its nucleotide sequence had been determined. The enzyme called ussurase cDNA encodes 699 nucleotides, namely 233 amino acids. Based on the ho-mology, the catalytic residues and disulfide bridges of ussurase were deduced as follows: catalytic residues, His40, Asp85 and Ser179; and disulfide bridges, Cys7-Cys138, Cys25 -Cys41, Cys73 - Cys231, Cys117 - Cys185, Cys149 - Cys164 and Cys175- Cys200.

关 键 词：长白蝮蛇 类凝血酶基因 克隆 序列分析 

分 类 号：Q959.62[生物学—动物学]