用低温取代法研究金鱼胚胎在低温下的超微结构图象  

A STUDY ON THE ULTRASTRUCTURE IMAGES OF GOLD FISH EMBRYOS AT LOW TEMPERATURES BY CRYOSUBSTITUTION

作　　者：俞国新[1] 任禾盛[1] 华泽钊[1] 陈锡浩[1] 王奇凤[1] 周美云[2] 

机构地区：[1]上海机械学院 [2]中科院上海细胞生物学研究所

出　　处：《上海机械学院学报》1991年第1期73-76,共4页

基　　金：国家自然科学基金

摘　　要：本文用低温取代法,研究了金鱼胚胎经两种不同的降温程序冷却到液氮温度后的超微结构图象。研究表明:若金鱼胚胎置入低浓度的抗冻剂溶液,慢速降温到-20℃后再快速浸入液氮,胚胎中有许多大的冰晶形成。这些大冰晶严重地破坏了金鱼胚胎的超微结构。而若将金鱼胚胎置入高浓度的玻璃化溶液中快速浸入液氮,胚胎外溶液可实现玻璃态固化。虽然胚胎内部仍有许多小的冰晶形成,但其体积比慢速降温时小得多,它们对胚胎细胞结构的影响也较小。In the present paper, the ultrastructure images of gold fish (carassius auratus) embryos cooled to LN_2 (liquid nitrogen) temperaturer by two ways are investigated by cryosubstitution. The main conclusions are: 1. When fish embryos in low cryoprotectant concentration solutions are cooled to -20℃ at slow cooling rates and then quenched to LN_2, many large ice-crystals which seriously damage the ultrastructure of the fish embryos are formed inside the embryos. 2. When fish embryos in high concentration vitrification solutions are directly quenched to LN_2, the extra-embryo solutions can be vitrified. Although many ice-crystals are still formed inside the embryos, they are much smaller than those in the slow cooling. The damage of small ice-crystals to the ultrastructure of fish embryos is much weaker than that of large ice-crystals.

关 键 词：低温取代法 金鱼 胚胎 超微结构 

分 类 号：Q954.4[生物学—动物学]