山荷降脂方对CCl_4诱导的小鼠急性肝损伤的保护作用  

Protective effects of Shanhejiangzhi decoction on acute liver injury

作　　者：龚峻梅[1] 朴英杰[1] 陈素云[2] 尹华清[3] 

机构地区：[1]中国人民解放军第一军医大学中心实验室 [2]中国人民解放军第一军医大学中医系 [3]中国人民解放军91821部队

出　　处：《暨南大学学报（自然科学与医学版）》2001年第6期71-74,共4页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的 :探讨山荷降脂方对CCl4 诱导的小鼠急性肝损伤的保护作用。方法 :用腹腔注射CCl4 法制作小鼠肝损伤模型。实验动物随机分为①正常对照组 ;②假肝损伤组 ;③肝损伤组 ;④生理盐水 +肝损伤组 ;⑤山荷降脂方 (低剂量 ) +肝损伤组 ;⑥山荷降脂方 (中剂量 ) +肝损伤组 ;⑦山荷降脂方 (高剂量 ) +肝损伤组。末次给药后 0 .5～ 1h ,腹腔注射质量分数为 0 .12 %的CCl4 花生油溶液(剂量为 0 .0 1mL/g) ,禁食 16h后处死。取肝脏 ,进行HE染色 ;球后取血 ,离心血清 ,测谷丙转氨酶、谷草转氨酶活性。结果 :(1)谷丙转氨酶、谷草转氨酶活性 :⑤、⑥、⑦组与③组比较有显著性差异 (P=0 .0 0 0 1) ,⑤、⑥、⑦组两两之间也有显著性差异 (P =0 .0 0 0 1)。 (2 )HE染色 ,光镜下观察肝脏组织病理学变化 ,⑥、⑦组肝损伤程度与③组比较 ,有显著性差异 (P <0 .0 1;P <0 .0 0 1) ,⑤、⑥组与⑦组也有显著性差异 (P <0 .0 0 1,P <0 .0 5 )。结论 :山荷降脂方对急性肝损伤具有呈量效关系的保护作用。Aim: To investigate the protective effects of Shanhejiangzhi decoction(SHJZD) on acute liver injury induced by carbon tetrachloride. Methods: The experimental models of acute liver injury were made by intraperitoneal injection of carbon tetrachloride(CCl 4). Mice were randomly divided into ①Control group; ②peanut oil group; ③CCl 4 group; ④physiological saline+CCl 4 group; ⑤SHJZD(lower dose)+CCl 4 group; ⑥SHJZD(moderate dose)+CCl 4 group; ⑦SHJZD(higher dose)+CCl 4 group. 30 min～1 h after the latest intragastric injection of SHJZD or physiological saline,0.12% carbon tetrachloride peanut oil solution were intraperitoneally injected to mice at a dose of 0.01 mL/g body weight, After mice were fasted for 16 h, animals were sacrificed. The severity of liver injury was assessed by histopathological examination and changes in GPT and GOT in the sera. Results: ①The activity of serum GPT or GOT in 5?6?7 group was much lower than in 3 group (P=0.000 1),there was statistic significant difference among 5?6?7 group (P=0.000 1). ②The histopathological results under microscope in 3 group were much serious than that in 6?7 group(P<0.01; P<0.001). Compared 5?6 group with 7 group, there was statistic difference (P<0.001;P<0.05). Conclusion: SHJZD has protective effects on acute liver injury in a dose-dependent manner.

关 键 词：山荷降脂方 急性肝损伤 四氯化碳 CCL4 小鼠 治疗 

分 类 号：R289.5[医药卫生—方剂学] R256.4[医药卫生—中药学]