RGD三肽液相合成的研究  

Study on the Liquid Phase Synthesis of RGD Tripeptide

作　　者：张倩[1] 张明智[1] 张成海[1] 丁伟[1] 

机构地区：[1]四川大学高分子材料科学与工程系,四川成都610065

出　　处：《四川大学学报（工程科学版）》2001年第6期78-81,共4页Journal of Sichuan University (Engineering Science Edition)

摘　　要：由N -保护的精氨酸、C -保护的甘氨酸采用混合酸酐法缩合成RG二肽 ,再与天冬氨酸在液相中合成RGD三肽。探讨了成酐和碱性试剂 ,反应温度 ,甘氨酸和天冬氨酸羧基保护等对缩合反应的影响。TLC跟踪反应与纯化全过程。以精氨酸计RGD三肽收率为 6 1.4 % ,HPLC确认其纯度为 88.6 6 %。The RG Dipeptides were condensated from N-protected Arg and C-protected Gly by mixed anhydrides method. Then Asp and RG Dipeptides were used synthesize RGD Tripeptides in liquid phase. The influencing factors of condensation reaction were investigated such as anhydrides, base agent, temperature, carboxyl group protected of Gly and Asp. TLC method follows the track of reaction and purification. The yield of RGD was 61.4% for Arg. The purity was 88.66% by HPLC.

关 键 词：RGD活性三肽 羧基保护 混合酸酐法 液相合成 抗血栓作用 精氨酸 甘氨酸 

分 类 号：R972.9[医药卫生—药品] R914.5[医药卫生—药学]