利用定量PCR方法研究对虾白斑杆状病毒感染与发病的关系  

Research of the Relation of White Spot Bacilliform Virus Infective Level and Disease Outbreak in Cultured Shrimp by Quantitative PCR

作　　者：徐丽美[1] 杨丰[1] 

机构地区：[1]国家海洋局第三海洋研究所,厦门361005

出　　处：《高技术通讯》2001年第12期9-11,共3页Chinese High Technology Letters

基　　金：86 3计划 ( 819 0 2 0 4) ;国家高新技术应用发展资助项目

摘　　要：收集了 6批共 134尾不同感染程度的对虾样品 ,测定其WSBV含量。检测结果表明 ,无病症的对虾样品每毫克组织所携带的病毒量低于 10 3 个病毒粒子 ,有病灶的对虾样品每毫克组织所携带的病毒量均高于 10 3 个病毒粒子 ,根据这一结果 ,初步确定了疾病爆发的危险临界值为每毫克组织含 10 3 个病毒粒子。In this report, 134 shrimps in various infectious degree were collected, and the amount of virus in each sample was determined by quantitative PCR. The result shows that shrimps without any symptom carry less than 10 3 virus particles per microgram tissue, while those with obvious symptom carry more than the amount. So 10 3 virus particles per microgram tissue would be the critical point for the shrimp virus disease outbreak.

关 键 词：对虾斑杆状病毒 WSBV 定量PCR 临界值 感染程度 发病学 

分 类 号：S945.1[农业科学—水产养殖]