RSV下呼吸道感染患儿辅助性T细胞亚群Th1/Th2及相关因子的研究  

The study of T-helper cell subpopulation Th1/Th2 and cytokine IFN-γ,IL-4 for infants with RSV LRTIs

作　　者：张艳敏[1] 雷春莲[1] 杨玉琮 阎辉[1] 成革胜[1] 

机构地区：[1]西安交通大学第一医院儿科,西安710061 [2]西安交通大学第一医院分子中心实验室

出　　处：《西安医科大学学报》2001年第6期561-563,共3页Journal of Xi'an Medical University(Chinese)

基　　金：陕西省卫生厅基金资助 (No .970 6 2 )

摘　　要：目的　探讨辅助性T淋巴细胞Th1 /Th2亚群及其相关细胞因子IFN γ、IL 4在呼吸道合胞病毒 (RSV)下呼吸道感染患儿发病机制中的作用。方法　用单克隆抗体双色免疫荧光FITC/PE双标记 ,流式细胞仪检测 3 0例RSV下呼吸道感染患儿急性期外周血单个核细胞(PBMCs)辅助性T淋巴细胞CD+4及其Th1亚群CD+4CD45RA+,Th2亚群CD+4CD45RO+的表达 ,并用ELISA方法同时检测患儿血清中细胞因子IFN γ、IL 4水平。同期检测 1 5例年龄、性别无差异的健康婴儿为对照。结果　RSV下呼吸道感染组患儿外周血辅助性T淋巴细胞CD+4明显低于对照组 (P <0 .0 5 ) ,Th1细胞CD+4CD45RA+明显下降 (P <0 .0 5 ) ,Th2细胞CD+4CD45RO+虽有下降 ,但与对照组相比无统计学差异 (P >0 .0 5 )。Th1 /Th2比值与对照组相比明显降低 (P <0 .0 5 )。RSV下呼吸道感染组患儿血清中IFN γ、IL 4水平均有降低 ,以IFN γ水平下降最为明显 (P <0 .0 1 ) ,IFN γ/IL 4比值降低 (P <0 .0 5 )。结论　婴幼儿RSV下呼吸道感染急性期的免疫功能紊乱主要表现为Th1及其功能下降 ;Th2及其功能相对增强 ,Th1 /Th2的失衡是导致该病发生Th2样反应的重要免疫机制。Objective To study the effect of T-helper cell CD + 4 subpopulation Th1/Th2 and cytokine IFN-γ,IL-4 in PBMCs for infants with RSV lower respiratory tract infections(LRTIs). Methods This research had measured 30 patients with RSV LRTIs CD + 4CD 45 RA +,CD + 4CD 45 RO + in PBMCs by flow cytometry analysis.And also measured IFN-γ,IL-4 level in serum and compared with control group.Results In RSV(+) infants' CD + 4 cells decreased greatly, and CD + 4CD 45 RA +,CD + 4CD 45 RO + all decreased,but CD + 4CD 45 RO + was not obvious compared with normal control, P > 0.05 .Th1/Th2 ratio decreased in RSV(+) infants. For IFN-γ,IL-4 level in serum, in RSV(+) group IFN-γ,IL-4 both decreased greatly.But IFN-γ level decreased greater than that of IL-4.IFN-γ/IL-4 ratio decreased in RSV(+) group.Conclusion In the PBMCs of RSV infants, there is disorder of immunology.Th1/Th2 unbalance occurs.Th2 takes high function relatively.The immunol reaction type is Th2 in RSV sever infection.

关 键 词：呼吸道合胞病毒 辅助性T细胞亚群 TH1/TH2 IL-4 IFN-Γ 

分 类 号：R725.603[医药卫生—儿科] R392.12[医药卫生—临床医学]