孕三烯酮和米非司酮对子宫内膜异位症患者异位内膜及腹腔液巨噬细胞凋亡的影响  

APOPTOSIS OF GESTRINONE AND MIFEPRISTON FOR THE ISOLATED ECTOPIC ENDOMETRIUM AND PERITONEAL FLUID MACROPHAGES FROM ENDOMETRIOSIS

作　　者：王云霞[1] 李亚里[1] 黄靖香[2] 昝世明 梁学颖[4] 

机构地区：[1]解放军总医院妇产科,北京100853 [2]解放军总医院骨科研究所 [3]解放军总医院病理科 [4]解放军总医院中心实验室

出　　处：《中国现代医学杂志》2001年第10期1-3,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金资助编号为 3 983 0 3 5 0

摘　　要：对培养的异位子宫内膜及腹腔液巨噬细胞分组加入孕三烯酮及米非司酮药物 ,观察其细胞生长情况及形态学变化 ,同时进行细胞凋亡率检测等研究 ,探讨子宫内膜异位症的临床治疗。方法 :对 12例患子宫内膜异位症妇女异位子宫内膜的离体组织及腹腔液进行细胞培养 ,在相同的生长期 ,分别加入一定剂量的孕三烯酮或米非司酮后 ,观察细胞的生长状况并用流式细胞仪检测其凋亡率。结果 :加入药物后 ,细胞的生长速度减慢 ,其凋亡率较未用药组明显增加 ,差异有显著性 (P <0 .0 5 ) ,而两种药物在同一种细胞 ,相同作用时间的凋亡率无统计学意义。结论 :孕三烯酮及米非司酮对异位子宫内膜细胞及腹腔液巨噬细胞有抑制作用 ,并且可以诱导异位子宫内膜细胞及腹腔液巨噬细胞的凋亡。Objective:to explore the methods of medicine treatment of endometriosis in clinical,we added various Gestrinone or Mifepriston to cells of ectopic endometrium and peritoneal fluid macrophages that we cultivated,meanwhile,we detected their apoptotic rate.Methods:We observed their growth and detected their apoptotic rate by FLOW CYTOMETER after the same growth term,the cell of isolated ectopic endometrium and peritoneal fluid macrophages that we cultivated from 12 patients with endometriosis.Results:The speed of cell growth was significantly decreased and their apoptotic rate was significantly increased compared with those of the control group(P<0.05),but no difference between Gestrinone and Mifepriston.Conclusions:Gestrinone and Mifepriston can restrain the ectopic endometrium and peritoneal fluid macrophages and induce them to apoptosis.The medicines play an important role in the treatment of endometriosis in clinical.

关 键 词：子宫内膜异位 异位细胞 腹腔液 巨噬细胞 孕三烯酮 米非司酮 凋亡率 

分 类 号：R711.71[医药卫生—妇产科学]