二仙汤加减对急性不完全性脑缺血大鼠脑保护作用的实验形态学研究  

EXPERIMENTAL MORPHOLOGY STUDY ON BRAIN PROTECTION WITH THE MODIFIED ERXIANTANG IN RATS WITH ACUTE INCOMPLETE CEREBRAL ISCHEMIA

作　　者：王洪海[1] 高慧英[2] 崔海庆[2] 李亚鲁[2] 李善刚[2] 石运芝[2] 

机构地区：[1]泰山医学院中医学教研室 [2]泰山医学院组织学与胚胎学教研室,泰安271000

出　　处：《解剖学杂志》2001年第6期547-551,共5页Chinese Journal of Anatomy

基　　金：山东省教育委员会资助课题 (NO J96K2 1 )

摘　　要：目的 :探讨二仙汤加减的脑保护作用及其作用机理。方法 :用中老年Wistar大鼠 ,采用双侧颈总动脉夹闭 2小时后 ,去夹再通的方法制作急性不完全性脑缺血再灌流模型。实验组大鼠造模前给予二仙汤加减 ,于血流再灌后 6h、2 4h、3d及 7d处死 ,对顶叶皮质及背侧海马进行形态学研究。结果 :实验组与对照组比较 ,光、电镜图象显示毛细血管、脑细胞渗出及细胞损伤明显减轻 ;海马CA1区细胞丢失明显减少 (P <0 .0 5 )。结论 :该方药有改善脑血液循环、减轻脑水肿和保护脑细胞的作用。Objective:To study the brain protection and the mechanism of the modified ERXIANTANG (EXT).Methods:Middle aged Wistar rats were used as acute incomplete ischmia.The model was made with the method of reperfusion after 2 h of the bilateral common carotid occlusion.The modified EXT was given before the rats were modeled.The rats were put to death 6 h,24 h,3 d and 7 d after the blood was reperfused.The cortex of parietal lobe and hippocampus were taken out for morphology study.Results:The light and electron micrograph showed that the leakage of the CP and cerebral cells alleviated obviously;The cells loss of CA1 in hippocampus decreased compared with control groups ( P <0.05).Conclusion:The modifid ERXIANTANG can prevent edema,improve blood circulation and protect the cerebral cells.

关 键 词：脑缺血 二仙汤加减 大鼠 脑保护作用 实验研究 

分 类 号：R289.5[医药卫生—方剂学]