肾移植长期存活受者外周血CC家族趋化因子及其受体表达的临床意义  

Expression and clinical implication of CC chemokines and receptor in long-term survived renal transplant recipients

作　　者：顾晓[1] 唐孝达[1] 肖家全[2] 周佩军[1] 凡杰[1] 谭建明[1] 徐达[1] 王祥慧[1] 

机构地区：[1]上海市第一人民医院泌尿外科,200080 [2]南京铁道医学院附属医院泌尿外科

出　　处：《中华泌尿外科杂志》2001年第12期709-711,共3页Chinese Journal of Urology

基　　金：国家自然科学基金项目资助 ( 39870 70 6 );上海市医学领先专业重点学科发展基金项目资助 ( 9830 0 5 )

摘　　要：目的　探讨肾移植长期存活受者不同免疫状态下外周血CC家族趋化因子及其受体的表达特征及临床意义。　方法　将 73例存活 3年以上的肾移植受者分为肾功能正常的对照组 (C组 )、低剂量免疫抑制组 (L组 )和慢性肾功能减退组 (D组 )。应用定量固相夹心酶联免疫吸附技术检测受者血浆中RANTES、单核细胞趋化蛋白 1(MCP 1)和巨噬细胞炎症蛋白 1α(MIP 1α)的含量 ,应用流式细胞技术检测外周血CCR5的表达率。　结果　L组的RANTES和MIP 1α含量及CCR5的表达率均明显低于C组 (P <0 .0 5 ) ,但三项指标在D、C组间差别无显著性意义 (P >0 .0 5 )。D组MCP 1含量显著高于C组 (P <0 .0 5 ) ,但在C、L组间差别无显著性意义 (P >0 .0 5 )。　结论　CC家族趋化因子及其受体表达与肾移植受者的免疫状态密切相关 。Objective To investigate the expression and clinical implication of CC chemokines and receptor in long surviving kidney grafted patients of different immune states. Methods 73 cases of recipients surviving more than 3 years were divided into three groups: control group of normal renal function(C group,n=32), group under low dosage of immunosuppressants(L group,n=20) and group with chronic allograft dysfounction(D group,n=21). The plasma RANTES、MCP 1 and MIP 1αwere detected by sandwich ELISA.The expression of CCR5 was determined by flow cytometry(FACS). Results Concentrations of RANTES and MIP 1α as well as expression rate of CCR5 in L group were lower than those of C group ( P <0.05),but there is no significant difference between D group and C group( P >0.05).Plasma level of MCP 1 was significantly higher in group D than that in group C( P <0.05),but the difference between group C and D was not significant( P >0.05). Conclusions Expression of CC chemokines and receptor closely correlated with the immune state of renal transplant recipients and could be valuable to estimate and monitor the immune state of patients.

关 键 词：肾移植 免疫学 趋化因子 受体 

分 类 号：R699.2[医药卫生—泌尿科学]