一种能产生紫杉醇类化合物内生真菌的分离  

Isolation of a Kind of Endophytic Fungus Which Can Produce Taxol Compounds

作　　者：邵爱娟[1] 林淑芳[1] 张思巨[1] 李晓明[1] 黄璐琦[1] 陈敏[1] 吴志刚[1] 戴如琴[1] 刘丽[1] 

机构地区：[1]中国中医研究院中药研究所生药室,北京100700

出　　处：《中国医学科学院学报》2001年第6期642-644,共3页Acta Academiae Medicinae Sinicae

摘　　要：目的从东北红豆杉TaxusCuspidataSiebetZucc树皮中分离能产生紫杉醇类化合物的内生真菌。方法采用无菌技术分离东北红豆杉树皮中的内生真菌,并采用高效液相色谱(HPLC)等方法对该真菌培养物进行分析。结果从东北红豆杉树皮中分离得到一种内生真菌,化学分析证明该真菌培养物内含有紫杉醇类化合物。结论东北红豆杉树皮中含有能产生紫杉醇类化合物的内生真菌。Objective To isolate a kind of endophytic fungus which can produce taxol compounds from the bark of Taxus Cuspidata Sieb et Zucc.Methods Endophytic fangus was isolated from the bark of Taxus Cuspidata Sieb et Zucc by aseptic techniques and the analysis of the culture fluid from the fermentation of fungus was carried out by high performance liquid chromatography(HPLC).Results Endophytic fungus was obtained from the bark of Taxus Cuspidata Sieb et Zucc and the chemical analysis showed that the culture of fungus contain taxol compounds.Conclusion Endophytic fungus which can produce taxol compounds exists in the bark of Taxus Cuspidata Sieb et Zucc.

关 键 词：东北 红豆杉 内生真菌 紫杉醇类化合物 高效液相色谱 抗癌药 

分 类 号：R979.1[医药卫生—药品] R931.6[医药卫生—药学]