无铅汽油新型添加剂甲基叔丁基醚的致突变性研究  

Study on Mutagenicity of Methyl Tertiary Butyl Ether of New Gasoline Additive

作　　者：袁东[1] 叶舜华[2] 周伟[2] 黄关麟[3] 

机构地区：[1]上海市疾病预防控制中心环境医学研究室,上海200336 [2]上海医科大学公共卫生学院环境卫生教研室 [3]中国石化南京炼油厂职工医院

出　　处：《中国公共卫生》2002年第2期129-130,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目 (39670 62 3);复旦大学公共卫生学院研究生课题

摘　　要：目的　研究甲基叔丁基醚 (MTBE)的致突变性及遗传毒性。方法　采用Ames试验检测MTBE的遗传毒性 ,并用单细胞凝胶电泳法检测国产MTBE大鼠经口亚慢性染毒后血液有核细胞的DNA的损伤状况。结果　Ames试验 (TA98和TA10 0菌株 )中 ,在加与不加S9的情况下 ,MTBE均未表现出明显的致突变性。染毒大鼠血液有核细胞DNA的断裂损伤也较对照组未见统计学差异。结论　本研究结果未发现MTBE对受试系统有致突变性。Objective To study the mutagenicity and genotoxicity of methyl tertiary butyl ether (MTBE).Methods Ames test was applied to detect its genotoxicity.The subchronic oral MTBE test,Single Cell Electrophoresis Assay (SCEA) were carried out to find the DNA damage in blood nucleolated cells of male Sprague-Dawley rats.Results MTBE did not show any mutagenic potential in Ames assay(TA98,TA10 strains)with or without rat liver-derived metabolic activation system(S-9),and also there was no significant difference in the DNA damage between the exposure and control group in subchronic test.Conclusion No mutagenicity appeared in this series of genotoxicigy tests.Further study must be performed on the effect of MTBE on the cell cycle to detect whether the MTBE had some degree of potential mutagenicity.

关 键 词：甲基叔丁基醚 致突变性 AMES试验 单细胞凝胶电泳 

分 类 号：R994.6[医药卫生—毒理学]