镍铬钴混合物对肾毒作用机理的研究  

Study on Mechanism of Kidney Toxicity of Mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride

作　　者：孙应彪[1] 朱玉真[1] 

机构地区：[1]兰州医学院公共卫生学系,730000

出　　处：《中国公共卫生》2002年第2期141-142,共2页Chinese Journal of Public Health

摘　　要：目的　探讨镍铬钴混合物致肾毒作用机理 ,为早期防治镍、铬、钴联合毒性所致的肾脏损伤提供理论依据。方法　模拟金川矿区矿石中镍、铬、钴平均含量百分比 (Ni∶Cr∶Co =0 2 6∶0 30∶0 0 2 ) ,连续 10日小鼠腹腔注射硫酸镍2 5mg/kg、重铬酸钾 0 14mg/kg、氯化钴 0 4 1mg/kg及其混合物 0 0 9mg/kg、0 17mg/kg染毒。制备肾小管细胞膜 ,采用定磷比色法检测肾小管细胞膜Na+ K+ -ATPase、Ca2 + -ATPase活性。结果　各单体和混合物明显抑制肾小管细胞膜Na+ K+ -ATPase、Ca2 + -ATPase活性 ,尤以混合物组显著 ,并且混合物毒性大于各单体毒作用。结论　混合物在体内可能产生联合毒性 ,抑制肾小管细胞膜ATPase活性而致肾脏损伤。Objective To study the mechanism of NiSO 4,K 2Cr 2O 7,CoCl 2 and the mixture to damage kidney of mice in order to provide theoretical evidence for preventing and curing joint toxicity of the mixture.Methods Several groups of mice were exposed respectively to NiSO 4,K 2Cr 2O 7,CoCl 2 at dose of 2.5mg/kg,0.14mg/kg,0.41mg/kg and a mixture of them at 0.09mg/kg,0.17mg/kg according to the ores percentage of average content (Ni∶Cr∶Co=0.26∶0.30∶0.02) in the Jinchuan area through intraperitoneal injection for 10 days.Renal tubular cell membrane were prepared to assess the activity of Na +.K +-ATPase,Ca 2+-ATPase with enzyme biochemical assay.Results The enzyme activity of Na +.K +-ATPase,Ca 2+-ATPase were inhibited obviously by NiSO 4,K 2Cr 2O 7,CoCl 2 and the mixture,and the mixture toxicity were stronger than that of NiSO 4,K 2Cr 2O 7,CoCl 2 lonely.Conclusion This could be the mixture that caused joint toxicity in the body of mice to inhabit the activity of Na +.K +-ATPase,Ca 2+-ATPase of renal tubular cell membrane so as to damage the kidney.However,the mechanism of joint toxicity on the mixture should be studied further.

关 键 词：硫酸镍 NiSO4 重铬酸钾 K2CrO7 氯化钴 CoCl2 膜毒理学 肾脏损伤 

分 类 号：R994.3[医药卫生—毒理学]