PAG内微量注射OFQ并同时电针对脊髓背角单胺类和氨基酸类递质释放的影响  

Effect of Microinjection of OFQ into PAG and Simultaneous Electroacupuncture on Release of Monoaminesand Amino Acids in Spinal Dorsal Horn

作　　者：杨芝兰[1] 张玉秋[1] 吴根诚[1] 

机构地区：[1]复旦大学医学院医学神经生物学国家重点实验室,针刺原理研究所上海200032

出　　处：《针刺研究》2001年第3期222-223,共2页Acupuncture Research

摘　　要：Our previous work demonstrated that microinjection of OFQ into PAG antagonized e lectroacupuncture (EA) analgesia; increased the release of Glu in spinal dorsal horn and decreased the release of GABA, 5 HT and NE; and significantly facilita t ed c fibre response and post discharge of WDR neurons of spinal dorsal horn in t he rats. The aim of current study is to observe the effect of microinjection of OFQ into PAG and simultaneous EA on the release of monoamines and Amino acids in spinal dorsal horn in the rats, by the methods of nucleus injection, micro dia lysis and HPLC EC. The results were as follows: After microinjection of OFQ into PAG and simultaneous EA, extracellular concentr ation of Glu was significantly decreased, whereas extracellular concentratio ns of GABA, 5 HT and 5 HIAA, NE and MHPG were significantly increas ed (P<0.05～0.01), indicating significant differences in comparison wi t h OFQ control group (microinjection of OFQ into PAG alone) in the corresponding phases (P<0.5～0.001). The results suggest there are opposite effects on the descending pain modulating system between OFQ in the brain and EA, so this may be one of the mechanisms of OFQ in the brain antagonizing EA analgesia.Our previous work demonstrated that microinjection of OFQ into PAG antagonized e lectroacupuncture (EA) analgesia; increased the release of Glu in spinal dorsal horn and decreased the release of GABA, 5 HT and NE; and significantly facilita t ed c fibre response and post discharge of WDR neurons of spinal dorsal horn in t he rats. The aim of current study is to observe the effect of microinjection of OFQ into PAG and simultaneous EA on the release of monoamines and Amino acids in spinal dorsal horn in the rats, by the methods of nucleus injection, micro dia lysis and HPLC EC. The results were as follows: After microinjection of OFQ into PAG and simultaneous EA, extracellular concentr ation of Glu was significantly decreased, whereas extracellular concentratio ns of GABA, 5 HT and 5 HIAA, NE and MHPG were significantly increas ed (P<0.05～0.01), indicating significant differences in comparison wi t h OFQ control group (microinjection of OFQ into PAG alone) in the corresponding phases (P<0.5～0.001). The results suggest there are opposite effects on the descending pain modulating system between OFQ in the brain and EA, so this may be one of the mechanisms of OFQ in the brain antagonizing EA analgesia.

关 键 词：PAG内注射 孤啡肽 电针 脊髓损伤 氨基酸 镇痛 痛觉调制 

分 类 号：R338.3[医药卫生—人体生理学] R245－0[医药卫生—基础医学]