中脑导水管周围灰质内催产素在电针镇痛中的作用及机制的实验研究  

Study on the Role and Mechanism of Oxytocin in PAG in Electroacupuncture Analgesia

作　　者：刘文彦[1] 白波[1] 刘玉红[1] 葛凤[1] 宋朝佑[2] 

机构地区：[1]济宁医学院生理学教研室,山东272113 [2]第二军医大学神经生物学教研室,上海200433

出　　处：《针刺研究》2001年第3期205-206,共2页Acupuncture Research

摘　　要：Objective: To explore the effect of oxytocin (OT) of periaqueducta l grey (PAG) of the midbrain on acupuncture analgesia and its relation with endo genous opiates.Methods: Potassium iron iontophoresis method was used to induce pain, tail flicking was considered as the pain index and the pai n threshold changes were detected before and after microinjection of oxytocin(OT , 2 ng/0.5 μL for each side), anti oxytocin serum (AOTS, 1 ng/0.5 μL), naloxo ne (Nx,5 ng/0.5 μL ), anti β endorphin serum(AEPS), anti metenkephalin seru m (AMEKS) and anti dynorphin A 1～13 serum(ADYNS) into PAG of rats. Results: After bilateral microinjection of OT , the analgesic effect of electroacupuncture (EA) increased significantly in com parison with that of microinjection of same dose of normal saline and presented a dose effect relation (P<0.01). Following microinjection of AOTS , the analgesic effect of EA decreased considerably in comparison with that of normal rabbit se rum (NRS, P<0.01). After microinjection of Nx, the analgesic effec t of EA also d eclined significantly compared with that of normal saline group (P <0.01). After microinjection of Nx and then OT, the effect of EA increased obviously compared with that of Nx+EA group(P<0.01). After microinjection of AEPS or and the n OT, the effect of EA also increased significantly in comparison with that of A EPS+EA and AMEKS+EA group (P<0.01). Following microinjection of AD YNS, the analg esic effect of EA had no significant changes in comparison with NRS group. After microinjection of ADYNS and then OT, the effect of EA increased significantly i n comparison with that of ADYNS+EA group while had no apparent changes in compar ison with that of OT+EA group (P>0.05). The results showed that OT in PAG plays an important role in the EA analgesia, and its effect is independ ent of endogen ous opiate peptides.Conclusion: In the central nervous system, m ultiple analge sic systems may exist simultaneously and OT participates in EA induced analgesic process.Objective: To explore the effect of oxytocin (OT) of periaqueducta l grey (PAG) of the midbrain on acupuncture analgesia and its relation with endo genous opiates.Methods: Potassium iron iontophoresis method was used to induce pain, tail flicking was considered as the pain index and the pai n threshold changes were detected before and after microinjection of oxytocin(OT , 2 ng/0.5 μL for each side), anti oxytocin serum (AOTS, 1 ng/0.5 μL), naloxo ne (Nx,5 ng/0.5 μL ), anti β endorphin serum(AEPS), anti metenkephalin seru m (AMEKS) and anti dynorphin A 1～13 serum(ADYNS) into PAG of rats. Results: After bilateral microinjection of OT , the analgesic effect of electroacupuncture (EA) increased significantly in com parison with that of microinjection of same dose of normal saline and presented a dose effect relation (P<0.01). Following microinjection of AOTS , the analgesic effect of EA decreased considerably in comparison with that of normal rabbit se rum (NRS, P<0.01). After microinjection of Nx, the analgesic effec t of EA also d eclined significantly compared with that of normal saline group (P <0.01). After microinjection of Nx and then OT, the effect of EA increased obviously compared with that of Nx+EA group(P<0.01). After microinjection of AEPS or and the n OT, the effect of EA also increased significantly in comparison with that of A EPS+EA and AMEKS+EA group (P<0.01). Following microinjection of AD YNS, the analg esic effect of EA had no significant changes in comparison with NRS group. After microinjection of ADYNS and then OT, the effect of EA increased significantly i n comparison with that of ADYNS+EA group while had no apparent changes in compar ison with that of OT+EA group (P>0.05). The results showed that OT in PAG plays an important role in the EA analgesia, and its effect is independ ent of endogen ous opiate peptides.Conclusion: In the central nervous system, m ultiple analge sic systems may exist simultaneously and OT participates in EA induced analgesic process.

关 键 词：中脑导水管周围灰质 催产素 电针 镇痛 动物实验 

分 类 号：R245－0[医药卫生—针灸推拿学] R338.3[医药卫生—中医临床基础]