二甲胂酸对人皮肤成纤维细胞DNA的损伤作用及缝隙连接通讯的影响  

Dimethylarsinic acid damages cellular DNA and inhibits gap junctional intercellular communication between human skin fibroblast cells

作　　者：邓芙蓉[1] 金昱[1] 王慧[1] 李新华[1] 郭新彪[1] 

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100083

出　　处：《北京大学学报（医学版）》2001年第6期497-500,共4页Journal of Peking University:Health Sciences

基　　金：美国中华医学基金专项人材基金 (ORF980 4)~~

摘　　要：目的 :研究二甲胂酸对人皮肤成纤维细胞DNA的损伤作用及细胞缝隙连接通讯的影响。方法 :分别采用单细胞凝胶电泳技术和细胞划痕染料标记示踪技术检测二甲胂酸对人皮肤成纤维细胞DNA的损伤作用及细胞缝隙连接通讯的影响。结果 :浓度为 0 .0 1～ 1.0mmol·L-1的二甲胂酸对人皮肤成纤维细胞的DNA损伤作用较强 ,出现较多的DNA断裂。 1.0mmol·L-1的二甲胂酸还可明显抑制细胞缝隙连接通讯 ,提示二甲胂酸可能有促癌活性。结论 :二甲胂酸的遗传毒性较强 ,同时二甲胂酸可能有促癌活性。Objective:To elucidate the mechanism of arsenic carcinogenesis Methods: Single-cell gel electrophoresis (SCGE) assay was used to detect the DNA damage in human skin fibroblast cells exposed to dimethylarsinic acid(DMAA), and the gas junctional intercellular communication (GJIC) between cells was detected by the scrape loading/dye transfer assay. Results: DMAA at concentrations of 0.01 mmol·L -1 -1.0 mmol·L -1 could induce DNA damage in a dose dependent manner, and GJIC between human skin fibroblast cells was significantly inhibited by DMAA at 1.0 mmol·L -1 . Conclusion: DMAA is genotoxic to human skin fibroblast cells. Furthermore, DMAA may also have tumor promoting activity.

关 键 词：二甲胂酸 药理学 DNA损伤 药物作用 细胞交流 细胞间接合部 

分 类 号：R995[医药卫生—毒理学] R965[医药卫生—药学]