纤维软骨细胞-胶原复合物修复半月板损伤的实验研究  

EXPERIMENTAL STUDY OF REPAIRING MENISCUS INJURY WITH FIBROCHONDROCYTECOLLAGEN CONSTRUCT

作　　者：祝云利[1] 荆鑫[1] 缪志和[1] 吴海山[1] 周维江[1] 

机构地区：[1]第二军医大学长征医院

出　　处：《解放军医学杂志》2002年第1期20-21,I001,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39770 743)

摘　　要：为了探讨纤维软骨细胞 胶原复合物对半月板损伤的修复作用 ,分离提取狗半月板纤维软骨细胞 ,体外扩增培养后种植于自制胶原模板上形成细胞 胶原复合物 ,将 18只狗随机分为 3组 ,在狗的外侧半月板作一楔形缺损 ,缺损内分别植入细胞 胶原复合物、胶原或不作处理 ,在不同时间取材进行大体和组织学观察。结果显示 ,植入的复合物形成与正常半月板相似的组织 ,胶原支架逐渐降解 ,而胶原对照组及空白对照组缺损区仅少部分修复或无修复。提示 ,这一新的组织工程学方法具有一定的指导意义及实用性。To investigate the repairing effect of fibrochondrocyte collagen construct in meniscus lesion, fibrochondrocytes were isolated and expanded in vitro , then seeded onto collagen construct .18 dogs with wedged shape lesion in the lateral meniscus were randomly divided into 3 groups: the lesion were filled with either cell collagen construct or collagen or untreated. The lateral meniscus was then examined grossly and histologically at different periods. The results showed that the lesions that had been filled with fibrochondrocyte collagen construct healed with fibrocartilage tissue. The results suggest that tissue engineering method is practical.

关 键 词：半月板损伤 细胞培养 胶原 生物医学工程 

分 类 号：R684[医药卫生—骨科学] R318[医药卫生—外科学]