肺表面活性物质蛋白质分离和生物活性作用  

ISOLATION OF PULMONARY SURFACTANT PROTEINS AND DETERMINATION OF ITS BIOLOGIC ACTIVITIES

作　　者：吴静怡[1] 徐志信[1] 沈慧卿[1] 孙家娥[1] 金勤立[1] 孔黄宽 罗松妹 

机构地区：[1]上海医科大学儿科研究所新生儿研究室 [2]中国科学院上海有机化学研究所

出　　处：《上海医科大学学报》1991年第6期421-425,共5页Journal of Fudan University(Medical Science)

摘　　要：肺表面活性物质蛋白质(8P)在肺表面活性物质(PS)的生理功能发挥中起着重要的作用。本文采用Sephadex LH-20柱层析分离纯化了牛肺PS,SDS-聚丙烯酰胺凝胶电泳分析SP分子量分别为30×10~3、10.37×10~3和5.08×10~3。并采用膜天平测定表面活性比较了含SP与不合SP的PS磷脂的生物活(忄牛),发现SP在PS吸附性、单分子膜形成性和稳定性中起着不可缺少的作用。Proteins play an important role in the physiologic function of pulmonary surfactant. Surfactant proteints were obtained by pulmonary lavage of the calf, and differentiated by sephadex LH-20 chromatography. SDS-polyacrylamide gel electrophoresis revealed the molecular weights of the surfactant proteins to be 30×103, 10.37×103, 5.08×103 respectively. Surface activities of surfactant phospholipids in the presence of or without surfactant proteins were assessed and compared.

关 键 词：肺表面活性物质 蛋白 PS 

分 类 号：R332.2[医药卫生—人体生理学]