聪圣胶囊对去皮层血管大鼠前脑脑源性神经营养因子及酪氨酸激酶mRNA作用的实验研究  

Experimental Study on Effect of Congsheng Capsule on Expression of Brain Derived Neurotrophic Factor and Tyrosinkinase mRNA in Forebrain of Rats after Cortical Devascularization

作　　者：司银楚[1] 朱培纯[1] 许红[1] 吴海霞[1] 

机构地区：[1]北京中医药大学解剖教研室,北京100029

出　　处：《中国中西医结合杂志》2001年第11期849-851,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：九五"国家科技攻关课题 (No .96 - 90 6 - 0 9- 0 3)

摘　　要：目的 :研究聪圣胶囊对去皮层血管模型大鼠前脑脑源性神经营养因子 (BDNF)mRNA、酪氨酸激酶 (trkB)mRNA作用及机理。方法 :建立去大鼠脑皮层血管模型 ,用原位杂交方法测定聪圣胶囊对其模型大鼠BDNFmRNA、trkBmRNA表达。结果 :发现去皮层血管后 ,聪圣胶囊对大鼠大脑皮质、海马中BD NFmRNA、trkBmRNA表达明显降低 ,海马CA1区下降最明显 ,大细胞基底核未见有BDNFmRNA的表达 ,而存在trkBmRNA的表达。结论 :聪圣胶囊通过增强BDNFmRNA、trkBmRNA的表达 ,促进BDNF及trkB蛋白的合成 ,挽救将要变性的神经元 。Objective: To study the effect and mechanism of Congsheng Capsule (CSC) on brain derived neurotrophic factor (BDNF) and tyrosinkinase (trkB) mRNA in forebrain of rats after cortical devascularization. Methods: The rat devascularization model was established, and expression of BDNF mRNA and trkB mRNA were determined by hybridization in situ method. Results: Expression of BDNF mRNA and trkB mRNA reduced obviously in the cerebral cortex and hippocampus of devascularization model, especially in hippocampal CA 1 subregion. There was trkB mRNA but no BDNF mRNA expression in the macrocyte basal nuclei (MBN). CSC significantly increased the decreased BDNF and trkB mRNA in cortex, hippocampus and MBN compared with placebo group. Conclusion: CSC could salvage the degenerating neurons and maintain their survival after cortical devascularization by increasing the expression of BDNF and trkB mRNA and improving the synthesis of BDNF mRNA and trkB protein.

关 键 词：聪圣胶囊 去皮层血管模型 脑源性神经营养因子 酪氨酸激酶 MRNA 

分 类 号：R285.5[医药卫生—中药学]