HCV包膜蛋白E2的表达及其抗原性的检测  

PROTEIN EXPRESSION AND ANTIGENICITY DETECTION OF HEPATITIS C VIRUS ENVELOPE PROTEIN E2

作　　者：史宣玲[1] 曹凤[1] 杜勇[2] 侯利华[2] 季阳[1] 王海涛[2] 

机构地区：[1]中国医学科学院中国协和医科大学输血研究所 [2]北京军事医学科学院微生物流行病研究所

出　　处：《解放军医学杂志》2002年第1期17-19,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39770 6 90 )

摘　　要：为研究丙型肝炎病毒 (HCV)包膜蛋白E2在原核细胞和真核细胞中的表达 ,并对表达蛋白的抗原性进行检测。将HCVE2区N端的一段基因分别克隆入原核表达载体pQE30和真核表达载体pEF1/HisC中进行E2蛋白的表达 ,采用ELISA和WB对表达蛋白的抗原性进行检测。结果在原核细胞和真核细胞中均表达了预期大小的蛋白 ,能同HCV感染者血清发生特异性反应。提示该段E2基因在原核表达系统和真核表达系统均能表达出具有抗原活性的蛋白 ,其中真核细胞表达的蛋白可被糖基化。Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.

关 键 词：C型肝炎样病毒属 病毒包膜蛋白质类 基因表达 HCV 丙型肝炎 

分 类 号：R512.63[医药卫生—内科学]