致病性小肠结肠炎耶氏菌DNA多态性  

DNA POLYMORPHISM OF YERSINIA ENTEROCOLITICA

作　　者：王军[1] 李跃旗[1] 赵蕊[1] 石建时[1] 王红旗[1] 王全立[1] 刘耀清[1] 马立人[1] 

机构地区：[1]解放军第302医院

出　　处：《解放军医学杂志》2002年第1期46-47,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：探讨致病性小肠结肠炎耶氏菌 (简称 :耶氏菌 )DNA分子遗传特征。用聚合酶链反应 (PCR)、DNA序列分析、随机扩增多态性DNA(RAPD)检测致病及非致病性耶氏菌 10株。耶氏菌 0∶3、0∶9、0∶5 ,2 7血清型均含有致病基因粘附侵袭位点 (ail) ,DNA指纹图谱也极其相似 ,与非致病型耶氏菌完全不同。然而 ,非致病性耶氏菌 0∶2 2血清型也含有ail基因 ,DNA指纹图谱在分子构像上部分与致病血清型相似。致病性耶氏菌具有相似的分子遗传特征 ,并表现为多态性。 0∶2 2血清型与致病性耶氏菌存在较近缘的种系进化关系。可能存在潜在致病性。To investigate the inherent characteristics of the pathogenic Yersinia enterocolitica. Polymerase chain reaction (PCR),analysis of DNA sequence,randomly amplified polymorphic DNA (RAPD) were used to study the pathogenic and nonpathogenic 10 strains of Yersinia enterocolitica. The Pathogenic Yersinia enterocolitica of serotyps 0∶3?0∶9 and 0∶5,27 were confirmed containing pathogenic gene of adherent invasion locus(ail) and their DNA fingerprints were much more similar,and it was also found that the serotype 0∶22 contained ail gene whose fingerprints was partly similar with the pathogenic ones in molecular structure. The pathogenic Yersinia enterocolitica strains were similar in the inherent and polymorphic. The serotype 0∶22 may have relativly evaluated association with pathogenic Yersinia enterocolitica, which is possible that it got potentially pathogenicity.

关 键 词：耳尔森氏菌 小肠结肠炎 聚合酶链反应 DNA序列分析 DNA指纹法 

分 类 号：R378[医药卫生—病原生物学]