血管内皮生长因子及血小板反应素在异位子宫内膜组织中的表达  

Expression of vascular endothelial growth factor and thrombospondin-1 in the ectopic and eutopic endometrium of women with endometriosis

作　　者：谭先杰[1] 郎景和[1] 刘东远[1] 沈铿[1] 冷金花[1] 孙大为[1] 刘珠凤[1] 朱兰[1] 许秀英[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,100730

出　　处：《中华妇产科杂志》2001年第12期727-730,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 (3 983 0 3 5 0 )

摘　　要：目的　探讨血管内皮生长因子 (VEGF)和血小板反应素 (TSP) 1在子宫内膜异位症 (内异症 )患者异位及在位子宫内膜组织中的表达及其意义。方法　采用逆转录聚合酶链反应 (RT PCR)和Northern杂交法 ,检测 38例内异症患者 5 2份异位子宫内膜组织 (腹膜红色病变 12份、卵巢子宫内膜异位囊肿 32份、宫骶韧带结节 8份 ) ,及 30份在位子宫内膜组织 (研究组 )中VEGFmRNA及TSP 1mRNA的阳性表达率。以 2 5例非内异症患者的 2 5份子宫内膜组织作为内异症患者在位内膜的对照(对照组 )。结果　腹膜红色病变VEGFmRNA表达水平较高 ,TSP 1mRNA表达水平较低 ;卵巢子宫内膜异位囊肿VEGFmRNA表达水平较低 ,而TSP 1mRNA表达水平较高。与对照组比较 ,研究组子宫内膜VEGFmRNA表达水平较高 ,而TSP 1mRNA表达水平较低。结论　内异症患者的异位子宫内膜中VEGF及TSP 1的表达 ,可能与其血管化程度有关 ;在位子宫内膜组织中VEGF及TSP 1表达水平的失衡 。Objective To detect the expression of vascular endothelial growth factor (VEGF) and thrombospondin (TSP) 1 mRNA in the ectopic and eutopic endometrium of patients with endometriosis Methods Thirty two specimens of ovarian endometrioma, 12 red peritoneal lesion, 8 uterosacral ligament nodule, and 30 matched eutopic endometrium were biopsied from 38 patients with endometriosis, another 25 specimens of endometrium were biopsied from women without endometriosis Reverse transcript polymerase chain reaction (RT PCR) and Northern Blot analysis were utilized to examine VEGF mRNA and TSP 1 mRNA expression and their relative levels in the above endometrium Results Among endometriotic lesions, red peritoneal lesion expressed higher level of VEGF and lower level of TSP 1, whereas ovarian endometrioma expressed lower level VEGF and higher level of TSP 1 Eutopic endometrium of women with endometriosis had higer level of VEGF expression and lower level of TSP 1 expression than women without endometriosis Conclusions The expression of VEGF and TSP 1 in endometriotic lesion appears to be associated with its neovalscualization extent The imbalance in expression of VEGF and TSP 1 in endometrium might be related to the development of endometriosis and its biological behavior

关 键 词：子宫内膜异位症 淋巴因子 内皮生长因子 血小板吸附蛋白类 VEGF TSP-1 

分 类 号：R711.71[医药卫生—妇产科学]