子宫内膜异位症病灶分泌的细胞因子与腹腔液微环境关系的研究  

Study on the correlation of the cytokine secreted by the endometrium focus and the microenvirment of the fleument fluid

作　　者：高颖[1] 刘义[1] 罗丽兰[1] 

机构地区：[1]华中科技大学同济医学院同济医院妇产科,武汉430030

出　　处：《中华妇产科杂志》2001年第12期723-726,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨子宫内膜异位症 (内异症 )病灶分泌的细胞因子与腹腔液之间的关系及其对生殖的影响。方法　对因内异症进行手术治疗或不孕症行腹腔镜检查的妇女 4 3例 ,其中内异症 2 4例 (内异症组 )及正常盆腔 19例 (对照组 ) ,采用单核细胞趋化实验分析、斑点杂交分析、酶链免疫吸附测定及胞浆游离钙浓度 ([Ca2 + ]i)测定等方法 ,测定两组患者的腹腔液、腹腔巨噬细胞及异位内膜细胞的特性 ,并比较两组腹腔液及异位内膜细胞对精子活动力及鼠胚胎卵裂率的影响。结果　内异症组及对照组患者腹腔液趋化单核细胞移动的距离分别为 (87 2± 9 4 ) μm、(5 1 9± 3 7) μm ,两组比较 ,差异有极显著性 (P <0 0 1) ;腹腔巨噬细胞数分别为 (1 4 7± 0 87)× 10 6/ml、(0 6 7± 0 73)× 10 6/ml(P<0 0 1) ;异位内膜细胞可表达低水平的单核细胞趋化蛋白 (MCP) 1[吸光度 (A)值为 18 34± 3 95 ],经白细胞介素 (IL) 1β及肿瘤坏死因子 (TNF) α刺激后 ,其分泌MCP 1mRNAA值分别为 5 6 84± 3 2 1、4 1 5 8± 2 4 8,刺激前后比较 ,差异有极显著性 (P <0 0 1) ;内异症组腹腔液及异位内膜细胞明显抑制精子的活动力和鼠胚的发育。结论　异位症病灶与腹腔液微环境相互影响 。Objective To investigate the correlation of endometriotic focus and peritoneal fluid as well as its effect on reproduction Methods Forty three women with surgical treatment or laproscope with infertility are divided into endometriosis group (24 cases) and normal pelvic group (control group 19 cases) The pathophysiology of patient′s peritoneal fluid, celiac macrophages and endometriotic cells are analyzed by chemotactic test on monocytes, dot blot hybridize, enzyme linked immunoadsorbent assay and the level of cytoplasmic [Ca 2+ ]i respectively Meanwhile, the influence of peritoneal fluid and endometriotic cells to sperm motivity as well as the effects to rat embryo cleavage are evaluated Results The monocyte moving distance induced by peritoneal fluid in endometriosis group Vs control is (87 2±9 4) μm Vs (51 9±3 7) μm ( P <0 01). The count of celiac macrophages (1 47±0 87)×10 6/ml Vs (0 67±0 73)×10 6/ml ( P <0 01), The basic expression level of monocyte chamotactic protein 1 (MCP 1) in endometriotic cells is (18 34±3 95) A . After exposured to IL 1β and tumor necrosis factor α, the OD of MCP 1mRNA is 56 84±3 21 A and 41 58± 2 48 A respectively ( P <0 01) Conclusion With the influence between endometriotic focus and microenvironment of peritoneal fluid,the level of cytokines expressed by celiac macrophages and endometriotic focus are enhanced,and it can interfere some process in reproduction

关 键 词：子宫内膜异位症 细胞活素类 微循环 腹腔液 细胞因子 

分 类 号：R711.71[医药卫生—妇产科学]